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盐度骤变对金钱鱼幼鱼鳃线粒体丰富细胞形态结构的影响

余焱方 刘鉴毅 冯广朋 赵峰 孙雪娜 王妤 邹雄 黄晓荣 李琪 孙艳秋 郑文明

余焱方, 刘鉴毅, 冯广朋, 赵峰, 孙雪娜, 王妤, 邹雄, 黄晓荣, 李琪, 孙艳秋, 郑文明. 盐度骤变对金钱鱼幼鱼鳃线粒体丰富细胞形态结构的影响[J]. 南方水产科学, 2021, 17(2): 60-69. doi: 10.12131/20200214
引用本文: 余焱方, 刘鉴毅, 冯广朋, 赵峰, 孙雪娜, 王妤, 邹雄, 黄晓荣, 李琪, 孙艳秋, 郑文明. 盐度骤变对金钱鱼幼鱼鳃线粒体丰富细胞形态结构的影响[J]. 南方水产科学, 2021, 17(2): 60-69. doi: 10.12131/20200214
Yanfang YU, Jianyi LIU, Guangpeng FENG, Feng ZHAO, Xuena SUN, Yu WANG, Xiong ZOU, Xiaorong HUANG, Qi LI, Yanqiu SUN, Wenming ZHENG. Effect of abrupt salinity change on morphology and structure of mitochondria-rich cells in Scatophagus argus[J]. South China Fisheries Science, 2021, 17(2): 60-69. doi: 10.12131/20200214
Citation: Yanfang YU, Jianyi LIU, Guangpeng FENG, Feng ZHAO, Xuena SUN, Yu WANG, Xiong ZOU, Xiaorong HUANG, Qi LI, Yanqiu SUN, Wenming ZHENG. Effect of abrupt salinity change on morphology and structure of mitochondria-rich cells in Scatophagus argus[J]. South China Fisheries Science, 2021, 17(2): 60-69. doi: 10.12131/20200214

盐度骤变对金钱鱼幼鱼鳃线粒体丰富细胞形态结构的影响

doi: 10.12131/20200214
基金项目: 中央级公益性科研院所基本科研业务费重点项目 (2019Z01);国家重点研发计划项目 (2019YFD 0900405)
详细信息
    作者简介:

    余焱方 (1994—),女,硕士研究生,研究方向为鱼类生理生态学。E-mail: 15955487288@163.com

    通讯作者:

    刘鉴毅 (1965—),男,研究员,从事珍稀濒危水生动物保护及水产生态养殖研究。E-mail: liujy@ecsf.ac.cn

  • 中图分类号: S 917.4

Effect of abrupt salinity change on morphology and structure of mitochondria-rich cells in Scatophagus argus

  • 摘要: 文章采用显微技术研究了盐度骤降 (20盐度组投入5盐度组)、盐度骤升 (20盐度组投入35盐度组)胁迫下,金钱鱼 (Scatophagus argus) 幼鱼鳃线粒体丰富细胞 (Mitochondria-rich cells, MRCs) 形态结构的变化。结果显示,低盐胁迫3 h MRCs长径 [(9.517±1.390) μm]和短径 [(7.150±1.448) μm]较对照组长径 [(7.317±0.986) μm]和短径 [(5.067±0.467) μm]显著增大 (P<0.05),高盐胁迫6 h MRCs数量显著增加。显微结果表明,胁迫24 h鳃丝处于渗透机制的主动调节阶段;超微结构观察发现鳃丝分化出2种类型的MRCs:1) Ⅰ型MRCs,椭圆形,胞核形状不规则,内脊发达,顶端开口处于关闭或开放状态,主要存在于低盐环境;2) Ⅱ型MRCs,圆形,胞核规则呈圆形,具有多个顶端开口。具不同形状的线粒体,a型线粒体着色较浅,呈短粗状;b型线粒体着色较深,呈小的颗粒状,主要存在于高盐环境。综上所述,金钱鱼幼鱼对盐度骤变产生应答反应机制,通过MRCs体积、数量、形状结构,以及线粒体的形状结构变化共同维持内环境稳定。
  • 图  1  5盐度组胁迫金钱鱼鳃的显微结构图

    a. 5盐度组3 h鳃丝整体图;b. 5盐度组3 h鳃丝部分放大图;c. 5盐度组6 h鳃丝部分放大图;d. 5盐度组12 h鳃丝部分放大图;e. 5盐度组24 h鳃丝部分放大图;f. 对照组 (20盐度) 鳃丝; g. 5盐度组96 h鳃丝部分放大图;MRCs. 线粒体丰富细胞;MC. 黏液细胞;BC. 血细胞;GL. 表示次级鳃小片直径;pl. 初级鳃小片;sl. 次级鳃小片;ecm. 在细胞外软骨质中;后图同此

    Figure  1.  Microscopic structure of S. argus gills at salinity of 5

    a. Whole picture of gill filament at salinity of 5 and at 3rd hour; b. Enlarged view of gill filament at salinity of 5 and at 3rd hour ; c. Enlarged view of gill filament at salinity of 5 and at 6th hour; d. Enlarged view of gill filament at salinity of 5 and at 12th hour; e. Enlarged view of gill filament at salinity of 5 and at 24th hour; f. Gill filament at salinity of 20 (Control group); g. Enlarged view of gill filament at salinity of 5 and at 96th hour; MRCs. Mitochondrion-rich cells; MC. Mucus cell; BC. Blood cell; GL. Secondary gill lamella diameter; pl. Primary gill lamella; sl. Secondary gill lamella; ecm. In extracellular cartilaginous bone. The same below.

    图  2  35盐度组胁迫金钱鱼鳃的显微结构图

    a. 35盐度组3 h鳃丝部分放大图;b. 35盐度组6 h鳃丝部分放大图;c. 35盐度组12 h鳃丝部分放大图;d. 35盐度组24 h鳃丝部分放大图;e. 35盐度组96 h鳃丝部分放大图;f. 对照组 (20盐度) 鳃丝部分放大图

    Figure  2.  Microscopic structure of S. argus gills at salinity of 35

    a. Enlarged view of gill filament at salinity of 35 and at 3rd hour; b. Enlarged view of gill filament at salinity of 35 and at 6th hour; c. Enlarged view of gill filament at salinity of 35 and at 12th hour; d. Enlarged view of gill filament at salinity of 35 and at 24th hour; e. Enlarged view of gill filament at salinity of 35 and at 96th hour; f. Control group, enlarged view of gill filament at salinity of 20

    图  3  5盐度组胁迫对金钱鱼鳃超微结构的影响

    a. 5盐度组3 h后鳃小片的超微结构; b. 5盐度组3 h后鳃的线粒体丰富细胞超微结构图,示Ⅰ型线粒体丰富细胞;c. 5盐度组6 h后鳃的线粒体丰富细胞超微结构图;d. 5盐度组12 h后鳃的线粒体丰富细胞超微结构图;e. 5盐度组24 h后鳃的线粒体丰富细胞超微结构图;f. 5盐度组48 h后鳃的线粒体丰富细胞超微结构图;g. 5盐度组96 h后鳃的线粒体丰富细胞超微结构图,示Ⅱ型线粒体丰富细胞结构;Ao. 顶端开口;Ap. 顶端小窝;MRCs. 线粒体丰富细胞;M. 线粒体;M1. a型线粒体;M2. b型线粒体;N. 细胞核;tn. 管网结构;lv. 光泡;JC. 紧密连接;N1. 形成色素的细胞核;h. 示a型线粒体;i. 对照组(20盐度组) 鳃超微结构整体图;Ts. 微细小管系统;后图同此

    Figure  3.  Effects of salinity 5 stress on ultrastructure of gill of S. argus

    a. Ultrastructure structure of gill lamella at salinity of 5 and at 3rd hour; b. Ultrastructure structure of MRCs of gill lamella at salinity of 5 and at 3rd hour, showing Type I mitochondrionrich cells; c. Ultrastructure structure of MRCs of gill lamella at salinity of 5 and a 6th hour; d. Ultrastructure structure of MRCs of gill lamella at salinity of 5 and at 12th hour; e. Ultrastructure structure of MRCs of gill lamella at salinity of 5 and at 24th hour; f. Ultrastructure structure of MRCs of gill lamella at salinity of 5 and at 48th hour; g. Ultrastructure structure of MRCs of gill lamella at salinity of 5 and at 96th hour, showing Type II mitochondrion-rich cells; Ao. Apical opening; Ap. Apical crypt; MRCs. Mitochondrion-rich cells; M1. Type a Mitochondrion; M2. Type b mitochondrion; N. Nucleus; tn. Tubular network; lv. Light vesicles; JC. Junctional complex; N1. Chromogenic nucleus; h. Showing Type a mitochondrion; i. Control group (salinity of 20), whole picture of gill ultrastructure; Ts. Microtubule system; the same below.

    图  4  35盐度组盐度骤降胁迫对金钱鱼幼鱼鳃超微结构的影响

    a. 35盐度组3 h后鳃的线粒体丰富细胞超微结构图;b. 35盐度组3 h后鳃的线粒体丰富细胞超微结构图放大图;c. 35盐度组6 h后鳃的线粒体丰富细胞超微结构图;d. 35盐度组12 h后鳃的线粒体丰富细胞超微结构图;e. 35盐度组24 h后鳃的线粒体丰富细胞超微结构图;f. 35盐度组48 h后鳃的线粒体丰富细胞超微结构图;g. 35盐度组96 h后鳃的线粒体丰富细胞超微结构图;h. 对照组 (20盐度) 鳃超微结构图;i. 对照组 (20盐度) 鳃超微结构整体图

    Figure  4.  Effects of salinity 35 stress on ultrastructure of gill of S. argus

    a. Ultrastructure structure of gill lamella at salinity of 35 and at 3rd hour; b. Enlarged view of ultrastructure structure of MRCs of gill lamella at salinity of 35 and at 3rd hour; c. Ultrastructure structure of MRCs of gill lamella at salinity of 35 and a 6th hour; d. Ultrastructure structure of MRCs ofgill lamella at salinity of 35 and at 12th hour; e. Ultrastructure structure of MRCs of gill lamella at salinity of 35 and at 24th hour; f. Ultrastructure structure of MRCs of gill lamella at salinity of 35 and at 48th hour; g. Ultrastructure structure of MRCs of gill lamella at salinity of 35 and at 96th hour; h. Control group (salinity of 20), ultrastructure structure of gill lamella; i. Control group (salinity of 20), whole picture of gill ultrastructure

    表  1  不同盐度下金钱鱼鳃线粒体丰富细胞长径、短径变化

    Table  1.   Changes of long and short diameters of MRCs in S. argus gill at different salinities

    时间
    t/h
    长径 Long diameter/μm 短径 Short diameter/μm
    20 (对照组)
    20 (Control group)
    5盐度组
    5 salinity group
    35盐度组
    35 salinity group
    20 (对照组)
    20 (Control group)
    5盐度组
    5 salinity group
    35盐度组
    35 salinity group
    3 7.317±0.986b 9.517±1.390c 6.933±0.831b 5.067±0.467b 7.150±1.448c 4.367±0.344ab
    6 8.033±0.873b 7.400±1.0.17b 4.783±1.020b 5.133±0.880b
    12 6.333±1.336b 6.267±0.723a 4.750±0.747b 3.850±0.638a
    24 6.567±0.582a 7.967±0.647b 4.433±0.723a 5.133±0.859b
    48 5.933±1.96a 6.917±1.187ab 4.017±0.360a 4.650±0.766ab
    96 7.650±0.403a 7.167±0.508ab 5.333±0.582a 5.967±0.588c
    注:表格中同一列参数后面有一个字母相同则无显著差异 (Duncan's法,P>0.05),反之,则差异显著 (P<0.05);后表同此 Note: If there is a same letter after the same column of numbers in the table, there is no significant difference (Duncan's, P>0.05); otherwise, the difference is significant (P<0.05). The same case in the following table.
    下载: 导出CSV

    表  2  不同盐度下金钱鱼次级鳃小片直径的变化

    Table  2.   Changes in diameter of secondary gill lamella of of S. argus at different salinities

    时间
    t/h
    20 (对照组)
    20 (Control group)
    5盐度组
    5 salinity group
    35盐度组
    35 salinity group
    310.567±1.217b12.783±1.603c21.333±2.510c
    68.317±1.312a10.750±2.157b
    128.933±2.554ab6.050±0.967a
    247.867±0.512a8.867±0.950b
    488.383±1.147a10.667±1.922b
    967.950±2.000a10.717±0.682b
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-10-15
  • 修回日期:  2020-11-15
  • 网络出版日期:  2021-01-08
  • 刊出日期:  2021-04-05

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