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肌醇对不同盐度条件下凡纳滨对虾幼虾生长和代谢的影响

何桂伦 易远名 杨奇慧 谭北平 迟淑艳 刘泓宇

何桂伦, 易远名, 杨奇慧, 谭北平, 迟淑艳, 刘泓宇. 肌醇对不同盐度条件下凡纳滨对虾幼虾生长和代谢的影响[J]. 南方水产科学. doi: 10.12131/20200186
引用本文: 何桂伦, 易远名, 杨奇慧, 谭北平, 迟淑艳, 刘泓宇. 肌醇对不同盐度条件下凡纳滨对虾幼虾生长和代谢的影响[J]. 南方水产科学. doi: 10.12131/20200186
HE Guilun, YI Yuanming, YANG Qihui, TAN Beiping, CHI Shuyan, LIU Hongyu. Effects of inositol on growth and metabolism for juvenile Litopenaeus vannamei at different salinities[J]. South China Fisheries Science. doi: 10.12131/20200186
Citation: HE Guilun, YI Yuanming, YANG Qihui, TAN Beiping, CHI Shuyan, LIU Hongyu. Effects of inositol on growth and metabolism for juvenile Litopenaeus vannamei at different salinities[J]. South China Fisheries Science. doi: 10.12131/20200186

肌醇对不同盐度条件下凡纳滨对虾幼虾生长和代谢的影响

doi: 10.12131/20200186
基金项目: 广东省社会发展计划项目“海洋高效微生态制剂的开发利用与产业化” (2013B021100017)
详细信息
    作者简介:

    何桂伦 (1993—),女,硕士研究生,研究方向为水产动物营养与饲料。E-mail: 2432526199@qq.com

    通讯作者:

    杨奇慧 (1978—),女,教授,博士,从事水产动物营养与饲料研究。E-mail: qihuiyang03@163.com

  • 中图分类号: S 963.3

Effects of inositol on growth and metabolism for juvenile Litopenaeus vannamei at different salinities

  • 摘要: 为研究肌醇对不同盐度 (4和28) 条件下凡纳滨对虾 (Litopenaeus vannamei) 幼虾生长、血清生化指标和非特异性免疫的影响,选取初始体质量为 (0.75±0.03) g的凡纳滨对虾为研究对象,以添加0、100、200、400、800和1 600 mg∙kg−1肌醇的6种等氮等脂饲料持续投喂10 周。结果表明,高盐度对虾终末体质量、增重率、成活率、特定生长率、蛋白质效率、蛋白质净利用率、总胆固醇含量、酸性磷酸酶、碱性磷酸酶、总超氧化物歧化酶和一氧化氮合酶显著高于低盐度 (P<0.05),饲料系数和水分则相反。饲料系数和总胆固醇含量随饲料中肌醇水平的升高而降低,终末体质量、增重率、成活率、特定生长率、蛋白质效率和蛋白质净利用率则相反,肌醇添加组的肝体比、酸性磷酸酶和碱性磷酸酶活性显著高于对照组 (P<0.05)。综上,饲料中添加肌醇可促进凡纳滨对虾的生长发育,提高其成活率和非特异性免疫力;低盐条件下凡纳滨对虾的生长发育和免疫力受抑制,肌醇水平不低于100 mg∙kg−1能使其生长发育恢复至高盐水平,不低于200 mg∙kg−1可在一定程度上提高其免疫力。
  • 表  1  实验饲料组成及营养水平 (干物质基础)

    Table  1.   Composition and nutrient levels of experimental diets (dry mass basis)

    原料
    Ingredient
    质量分数
    Mass fraction/%
    红鱼粉 Brown fish meal 16.00
    酪蛋白 Casein 28.00
    明胶 Gelatin 7.00
    高筋面粉 Bread flour 25.00
    磷脂 Phospholipid 2.00
    鱼油 Fish oil 2.00
    豆油 Soy oil 2.00
    磷酸二氢钙 Ca(H2PO4)2 1.50
    VC单聚磷酸酯 VC monopoly phosphate 0.02
    去肌醇维生素预混料 Vitamin premix 1.00
    矿物质预混料 Mineral premix 2.00
    胆碱 Choline 0.10
    微晶纤维素+肌醇 Microcrystalline cellulose + Inositol 13.38
    合计 Total 100.00
    营养成分 Nutritional ingredient
     粗蛋白 Crude protein 40.00
     粗脂肪 Crude fat 6.50
    注:① 每千克饲料中维生素预混料各组分质量 (mg):泛酸钙61.00,氰钴胺素0.10,纤维素1389.65,核黄素25.00,烟酸201.00,VD 120.00,叶酸 6.25,VA 10,VE 99.00,生物素2.50,VK 10.00,VB1 25.50,吡哆醇50.00。② 每千克饲料中矿物质预混料各组分质量 (mg):NaSeO3 4.00,KIO4 0.06,CuSO4·5H2O 39.68,MnSO4·7H2O 0.24,MgSO4·H2O 24.86,Ca(PO4)2 160.00,KCl 30.66,沸石粉1648.38,柠檬酸铁 27.42,CoCl2·6H2O 8.14,ZnSO4·7H2O 56.56。 Note: ① Content of each component of vitamin premix per kg of feed (mg): calcium pantothenate 61.00, cyanocobalamine 0.10, cellulose 1389.65, riboflavin 25.00, niacin 201.00, VD 120.00, folic acid 6.25, VA 10, VE 99.00, biotin 2.50, VK 10.00, VB1 25.50, pyridoxine 50.00. ② Content of each component of mineral premix per kg of feed (mg): NaSeO3 4.00, KIO4 0.06, CuSO4·5H2O 39.68, MnSO4·7H2O 0.24, MgSO4·H2O 24.86, Ca(PO4)2 160.00, KCl 30.66, zeolite powder 1648.38, ferric citrate 27.42, CoCl2·6H2O 8.14, ZnSO4·7H2O 56.56.
    下载: 导出CSV

    表  2  肌醇对凡纳滨对虾幼虾生长性能的影响

    Table  2.   Effects of inositol on growth performance for juvenile L. vannamei

    组别
    Group
    终末体质量
    FW/g
    增重率
    WG/%
    成活率
    SR/%
    特定生长率
    SGR/%
    饲料系数
    FC
    蛋白质效率
    PER
    每个处理的平均值 Individual treatment means
     LJ0 5.75±0.77e 672.19±106.85e 63.33±7.63d 2.91±0.19g 6.77±1.80a 0.32±0.09g
     LJ1 9.16±0.52cd 1 129.56±68.51cd 69.16±12.33cd 3.58±0.08ef 3.51±0.76b 0.59±0.11f
     LJ2 9.14±0.19cd 1 126.01±28.16cd 85.00±5.00ab 3.58±0.03ef 2.70±0.14bcd 0.76±0.03e
     LJ3 9.90±0.46c 1 229.81±61.35c 92.50±4.33a 3.69±0.06de 2.23±0.14cde 0.91±0.05cd
     LJ4 10.91±0.29b 1 358.14±39.26b 88.33±6.29ab 3.82±0.03cd 2.13±0.12cde 0.95±0.05c
     LJ5 11.54±0.27b 1 446.59±30.24b 93.33±2.88a 3.91±0.02bc 1.79±0.01de 1.10±0.01b
     HJ0 8.84±0.19d 1 079.98±42.62d 78.33±7.63bc 3.45±0.02f 3.01±0.27bc 0.63±0.05f
     HJ1 9.45±0.37cd 1 163.08±85.80cd 90.00±4.33a 3.62±0.09e 2.45±0.28cde 0.81±0.09de
     HJ2 11.02±0.17b 1 369.97±39.30b 92.50±2.50a 3.79±0.05cd 1.94±0.05cde 0.98±0.02c
     HJ3 11.45±0.47b 1 432.89±115.96b 93.33±2.88a 3.91±0.07bc 1.81±0.09de 1.13±0.05b
     HJ4 12.49±0.17a 1 570.03±38.17a 89.16±1.44a 4.02±0.03ab 1.65±0.04de 1.15±0.03b
     HJ5 12.96±0.13a 1 631.10±35.36a 95.83±1.44a 4.07±0.02a 1.42±0.03e 1.34±0.02a
    单个因子的平均值 Means of single factor
     J0 7.29E 876.09E 70.83B 3.18D 4.89A 0.47E
     J1 9.30D 1 146.32D 79.58B 3.60C 2.98B 0.70D
     J2 10.08C 1 247.99C 88.75A 3.68C 2.32BC 0.87C
     J3 10.68B 1 331.35B 92.91A 3.80B 2.02C 1.02B
     J4 11.70A 1 464.09A 88.75A 3.92A 1.89C 1.05B
     J5 12.25A 1 538.84A 94.58A 3.99A 1.60C 1.22A
     L 9.40x 1 160.38x 81.94x 3.58x 3.19y 0.77x
     H 11.04y 1 374.51y 89.96y 3.81y 2.04x 1.01y
    双因素分析PP-value of Two-way ANOVA
     盐度因素 Salinity factor 0.000 0.000 0.000 0.000 0.000 0.000
     肌醇因素 Inositol factor 0.000 0.000 0.000 0.000 0.000 0.000
     交互作用 Interaction 0.002 0.002 0.026 0.000 0.000 0.652
    注:同列的不同小写字母表示差异显著 (P<0.05);A、B、C、D、E代表饲料因素;x、y代表盐度因素;下表同此。 Note: Values with different lowercase letters within the same column have significant difference (P<0.05). The diet factor is represented by A, B, C, D and E; the salinity factor is represented by x and y, the same below.
    下载: 导出CSV

    表  3  肌醇对凡纳滨对虾幼虾体成分的影响 (干物质基础)

    Table  3.   Effects of inositol on body composition for juvenile L. vannamei (dry matter basis)

    组别
    Group
    肝体比
    HSI
    水分
    Moisture/%
    粗蛋白
    Crude protein/%
    粗脂肪
    Crude lipid/%
    粗灰分
    Ash/%
    蛋白质净利用率
    NPU/%
    每个处理的平均值 Individual treatment means
     LJ0 3.15±0.11c 76.63±0.78abc 75.57±0.20 12.46±1.58 13.27±0.48 4.49±1.32g
     LJ1 3.63±0.24ab 77.64±1.89a 75.91±1.86 11.49±0.25 13.15±0.42 8.75±1.92f
     LJ2 3.82±0.1ab 76.83±0.71ab 76.37±1.10 10.42±1.75 13.24±0.25 10.88±0.65e
     LJ3 3.81±0.07ab 75.44±0.11bcde 76.03±1.63 11.78±2.43 12.98±0.49 13.10±1.02d
     LJ4 3.90±0.02a 75.92±0.13bcde 76.39±0.76 10.97±0.16 13.13±0.45 13.55±0.64d
     LJ5 3.64±0.10ab 76.14±0.71 bcd 76.33±0.48 11.13±0.84 12.92±0.04 16.38±0.07bc
     HJ0 3.45±0.13bc 74.77±0.50de 75.57±0.20 12.46±0.91 13.27±0.28 10.16±0.86ef
     HJ1 3.45±0.26bc 75.28±0.71cde 75.91±1.86 11.49±0.14 13.15±0.24 12.82±1.41d
     HJ2 3.51±0.33bc 75.28±0.40cde 76.37±1.10 10.42±1.01 13.24±0.14 15.74±0.37bc
     HJ3 3.81±0.28ab 75.04±0.83de 76.03±1.63 11.78±1.40 12.98±0.28 15.27±0.82c
     HJ4 3.90±0.16a 75.56±0.37bcde 76.39±0.76 10.97±0.09 13.13±0.26 17.11±0.48b
     HJ5 3.61±0.20ab 74.60±0.38e 76.33±0.48 11.13±0.49 12.92±0.02 19.48±0.33a
    单个因子的平均值 Means of single factor
     J0 3.30D 75.70 75.57 12.46 13.271 7.32E
     J1 3.54C 76.46 75.91 11.49 13.153 10.79D
     J2 3.67ABC 76.05 76.37 10.42 13.244 13.31C
     J3 3.81AB 75.24 76.30 11.78 12.981 14.19BC
     J4 3.90A 75.74 76.39 10.97 13.131 15.33B
     J5 3.62BC 75.37 76.33 11.13 12.925 17.93A
     L 3.66 76.44y 76.10 11.37 13.12 11.19x
     H 3.62 75.09x 76.19 11.37 13.11 15.10y
    双因素分析PP-value of Two-way ANOVA
     盐度因素 Salinity factor 0.564 0.000 0.814 0.992 0.964 0.000
     肌醇因素 Inositol factor 0.001 0.111 0.740 0.089 0.372 0.000
     交互作用 Interaction 0.197 0.189 0.997 1.000 1.000 0.058
    下载: 导出CSV

    表  4  肌醇对凡纳滨对虾幼虾血清生化指标影响

    Table  4.   Effects of inositol on serum biochemical indexes for juvenile L. vannamei

    组别
    Group
    总蛋白质量浓度
    TP/(g∙L−1)
    甘油三酯浓度
    TG/(mmol∙L−1)
    总胆固醇浓度
    TC/(mmol∙L−1)
    葡萄糖浓度
    Glu/(mmol∙L−1)
    每个处理的平均值 Individual treatment means
     LJ0 61.80±8.09 0.45±0.04 1.63±0.36bc 0.74±0.09b
     LJ1 63.63±3.97 0.41±0.04 1.35±0.36bcd 0.89±0.11ab
     LJ2 65.00±8.75 0.42±0.02 1.38±0.18bcd 0.90±0.12ab
     LJ3 66.00±7.56 0.39±0.04 1.16±0.31cd 0.97±0.06ab
     LJ4 67.33±10.58 0.33±0.03 1.13±0.22cd 0.97±0.24ab
     LJ5 70.00±6.32 0.33±0.05 0.91±0.18d 1.36±0.94a
     HJ0 59.76±4.70 0.60±0.51 2.53±0.34a 0.85±0.07ab
     HJ1 59.66±8.63 0.46±0.06 1.90±0.45b 0.98±0.09ab
     HJ2 62.86±2.61 0.42±0.04 1.94±0.53b 1.04±0.14ab
     HJ3 69.80±6.10 0.42±0.05 1.81±0.40b 1.17±0.14ab
     HJ4 61.03±1.76 0.33±0.18 1.74±0.22bc 1.35±0.10a
     HJ5 64.30±8.65 0.34±0.02 1.40±0.29bcd 1.38±0.18a
    单个因子的平均值 Means of single factor
     J0 62.38 0.52 2.08A 0.79
     J1 63.52 0.43 1.63B 0.94
     J2 63.25 0.42 1.66B 1.13
     J3 68.57 0.41 1.49BC 1.17
     J4 64.83 0.33 1.43BC 1.00
     J5 63.05 0.33 1.15C 1.27
     L 65.62 0.39 1.26x 0.97
     H 62.90 0.43 1.89y 1.13
    双因素分析PP-value of Two-way ANOVA
     盐度因素 Salinity factor 0.254 0.476 0.000 0.140
     肌醇因素 Inositol factor 0.682 0.358 0.003 0.125
     交互作用 Interaction 0.573 0.960 0.928 0.491
    下载: 导出CSV

    表  5  肌醇对凡纳滨对虾幼虾部分非特异性免疫酶活的影响

    Table  5.   Effects of inositol on some non-specific immunoenzyme activities for juvenile L. vannamei

    组别
    Group
    酸性磷酸酶
    ACP/(U∙100 mL−1)
    碱性磷酸酶
    AKP/(U∙100 mL−1)
    总超氧化物歧化酶
    T-SOD/(U∙mL−1)
    一氧化氮合成酶
    NOS/(U∙100 mL−1)
    每个处理的平均值 Individual treatment means
     LJ0 5.75±0.19cd 1.85±0.04f 280.13±56.22de 22.61±0.66f
     LJ1 6.02±0.11bcd 1.88±0.04ef 285.73±37.71cde 22.48±1.85f
     LJ2 6.37±0.42b 2.11±0.05d 248.77±23.40e 24.48±1.14def
     LJ3 6.24±0.08b 2.03±0.03d 249.16±2.88e 26.62±3.03def
     LJ4 6.34±0.11b 2.02±0.19de 254.07±4.59e 24.09±0.40ef
     LJ5 6.05±0.18bc 2.43±0.09bc 263.80±10.85e 26.28±2.34def
     HJ0 5.58±0.33d 2.34±0.06c 282.26±12.58de 28.69±1.29cd
     HJ1 6.14±0.09bc 2.69±0.09a 319.24±16.30bcd 30.96±3.42c
     HJ2 6.83±0.39a 2.39±0.06bc 335.03±13.24ab 35.17±3.41b
     HJ3 6.86±0.26a 2.53±0.07b 367.56±22.07a 47.56±4.17a
     HJ4 6.33±0.28b 2.46±0.10bc 326.23±7.31abc 31.56±1.58bc
     HJ5 6.14±0.27bc 2.47±0.07bc 340.85±10.30ab 27.79±0.55cde
    单个因子的平均值 Means of single factor
     J0 5.66C 2.09C 281.19 25.65C
     J1 6.08B 2.28B 302.48 26.72C
     J2 6.60A 2.25B 291.90 29.82B
     J3 6.55A 2.28B 308.36 37.09A
     J4 6.33AB 2.24B 290.15 27.82BC
     J5 6.09B 2.45A 302.32 27.03BC
     L 6.12x 2.05x 263.61x 24.42x
     H 6.31y 2.48y 328.52y 33.62y
    双因素分析PP-value of Two-way ANOVA
     盐度因素 Salinity factor 0.038 0.000 0.000 0.000
     肌醇因素 Inositol factor 0.000 0.000 0.379 0.000
     交互作用 Interaction 0.103 0.000 0.004 0.000
    下载: 导出CSV
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  • 收稿日期:  2021-06-26
  • 修回日期:  2021-09-07
  • 录用日期:  2021-10-11
  • 网络出版日期:  2021-10-27

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