王成龙, 关文志, 李永强, 刘峰. 17β-雌二醇诱导黄颡鱼雌性化的研究[J]. 南方水产科学, 2020, 16(3): 25-30. DOI: 10.12131/20200001
引用本文: 王成龙, 关文志, 李永强, 刘峰. 17β-雌二醇诱导黄颡鱼雌性化的研究[J]. 南方水产科学, 2020, 16(3): 25-30. DOI: 10.12131/20200001
WANG Chenglong, GUAN Wenzhi, LI Yongqiang, LIU Feng. Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco[J]. South China Fisheries Science, 2020, 16(3): 25-30. DOI: 10.12131/20200001
Citation: WANG Chenglong, GUAN Wenzhi, LI Yongqiang, LIU Feng. Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco[J]. South China Fisheries Science, 2020, 16(3): 25-30. DOI: 10.12131/20200001

17β-雌二醇诱导黄颡鱼雌性化的研究

Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco

  • 摘要: 该研究通过对17β-雌二醇 (E2) 的投喂方式和投喂时间进行比较,探索了XY全雄黄颡鱼 (Pelteobagrus fulvidraco) 的雌性化方法,进而建立3个家系 (XY♀×XY♂),结合性别分子标记对黄颡鱼雌性化诱导的成活率和性逆转率进行了对比研究。结果显示,卤虫 (Artemia sp.) 组从开口第1天进行诱导的性逆转率为75.20%,而从开口第5天进行诱导得不到发育好的雌性;从开口第1天投喂激素,饲料组的成活率与卤虫组无显著差异 (P>0.05),而性逆转率显著高于卤虫组 (P<0.05);分子标记Y1和Y2相结合能够准确鉴定黄颡鱼的遗传性别;对照组中3个家系的成活率和各个基因型性别比例均无显著差异(P>0.05),成活率为51.00%~56.67%,XX雌性占比为90.91%~97.44%,XY雄性占比为94.56%~95.96%,而YY则为雄性;诱导组中3个家系的成活率和性逆转率均无显著差异(P>0.05)。结果表明,采用含50 mg·kg−1 E2的微颗粒饲料从开口第1天开始诱导30 d,并运用遗传性别与表型性别鉴定相结合的方法能够有效筛选获得发育良好的XY/YY雌性黄颡鱼。

     

    Abstract: In this study, we compared the feeding method and feeding time of 17β-estradiol to explore the feminization method of XY Pelteobagrus fulvidraco, and then established three families (XY♀×XY♂) to compare the survival rate and sex reversal rate with sex molecular. The results show that the females in Artemia sp. group could not be induced to develop well from the 5th day of opening, but the induced reversal rate was 75.20% on the 1st day of opening, the survival rate induced  from the 1st day of opening was not significantly different from that of Artemia sp. group, while the sex reversal rate was significantly higher than that of Artemia group; the combination of molecular markers Y1 and Y2 could identify the genetic sex of P. fulvidraco accurately; there was no significant difference in the survival rate and sex ratio of each genotype among the three families in the control group, and the survival rate was 51.00%−56.67%. The proportion of XX female and XY male individuals were 90.91%−97.44% and 94.56%−95.96%, respectively. There was no significant difference in the survival rate and sex reversal rate among the three families in the induction group. The result shows that using 50 mg·kg−1 E2 mixed with micro pellet feed to induce 30 d from the 1st day of opening can screen out well-developed female XY/YY P. fulvidraco effectively by combination of genetic and phenotypic sex identification.

     

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