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17β-雌二醇诱导黄颡鱼雌性化的研究

王成龙 关文志 李永强 刘峰

王成龙, 关文志, 李永强, 刘峰. 17β-雌二醇诱导黄颡鱼雌性化的研究[J]. 南方水产科学, 2020, 16(3): 25-30. doi: 10.12131/20200001
引用本文: 王成龙, 关文志, 李永强, 刘峰. 17β-雌二醇诱导黄颡鱼雌性化的研究[J]. 南方水产科学, 2020, 16(3): 25-30. doi: 10.12131/20200001
Chenglong WANG, Wenzhi GUAN, Yongqiang LI, Feng LIU. Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco[J]. South China Fisheries Science, 2020, 16(3): 25-30. doi: 10.12131/20200001
Citation: Chenglong WANG, Wenzhi GUAN, Yongqiang LI, Feng LIU. Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco[J]. South China Fisheries Science, 2020, 16(3): 25-30. doi: 10.12131/20200001

17β-雌二醇诱导黄颡鱼雌性化的研究

doi: 10.12131/20200001
基金项目: 2018年度上海市市级农口系统青年人才成长计划项目〔沪农青字 (2018) 第3—4号〕
详细信息
    作者简介:

    王成龙 (1989—),男,硕士,助理工程师,从事水产动物遗传育种研究。E-mail: 836597116@qq.com

    通讯作者:

    刘 峰 (1975—),男,副研究员,从事水产动物遗传育种研究。E-mail: lf6475@sina.com

  • 中图分类号: S 917.4

Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco

  • 摘要:

    该研究通过对17β-雌二醇 (E2) 的投喂方式和投喂时间进行比较,探索了XY全雄黄颡鱼 (Pelteobagrus fulvidraco) 的雌性化方法,进而建立3个家系 (XY♀×XY♂),结合性别分子标记对黄颡鱼雌性化诱导的成活率和性逆转率进行了对比研究。结果显示,卤虫 (Artemia sp.) 组从开口第1天进行诱导的性逆转率为75.20%,而从开口第5天进行诱导得不到发育好的雌性;从开口第1天投喂激素,饲料组的成活率与卤虫组无显著差异 (P>0.05),而性逆转率显著高于卤虫组 (P<0.05);分子标记Y1和Y2相结合能够准确鉴定黄颡鱼的遗传性别;对照组中3个家系的成活率和各个基因型性别比例均无显著差异(P>0.05),成活率为51.00%~56.67%,XX雌性占比为90.91%~97.44%,XY雄性占比为94.56%~95.96%,而YY则为雄性;诱导组中3个家系的成活率和性逆转率均无显著差异(P>0.05)。结果表明,采用含50 mg·kg−1 E2的微颗粒饲料从开口第1天开始诱导30 d,并运用遗传性别与表型性别鉴定相结合的方法能够有效筛选获得发育良好的XY/YY雌性黄颡鱼。

  • 图  1  分子标记Y1和Y2在正常黄颡鱼 (XX,XY) 中PCR扩增条带电泳图

    M. 标准物质2 000;后图同此

    Figure  1.  Electrophoresis of PCR amplified bands of molecular markers Y1 and Y2 in P. fulvidraco (XX, XY)

    M. Marker 2 000; the same below

    图  2  分子标记Y1和Y2在黄颡鱼家系 (XY♀×XY♂) 部分个体PCR扩增条带电泳图

    Figure  2.  Electrophoresis of PCR amplified bands of molecular markers Y1 and Y2 in some individuals of P. fulvidraco family (XY♀×XY♂)

    表  1  17β-雌二醇处理的两种饵料对黄颡鱼雄鱼 (XY) 成活率和性逆转率的影响

    Table  1.   Effects of two kinds of bait treated by E2 on survival rate and sex reversal rate of male P. fulvidraco (XY)

    指标
    Index
    卤虫组
    Artemia sp. group
    饲料组
    Feed group
    对照组
    Control group
    开口第几天投喂 Feeding on nth day of opening 1 5 1 1
    发育好 Well developed/尾 27.33±6.57a 0.00±0.00b 39.33±5.70a 0.00±0.00b
    发育差 Poorly developed/尾 7.33±1.33a 38.67±2.40b 0.00±0.00c 0.00±0.00c
    雄性 Male/尾 1.33±1.33a 8.00±1.15b 0.00±0.00a 79.67±3.18c
    成活率 Survivall rate/% 36.00±7.57a 46.67±2.40a 39.33±5.70a 79.67±3.18b
    性逆转率 Sex reversal rate/% 75.20±2.72a 0.00±0.00b 100.00±0.00c 0.00±0.00b
    注:同一行数字上的不同字母表示差异显著 (P<0.05) Note: The values with different letters within the same row indicate significant difference (P<0.05).
    下载: 导出CSV

    表  2  3个家系正常组的成活率及各个基因型性别比例统计

    Table  2.   Statistics of survival rate and sex ratio of each genotype in three normal families

    基因型及指标
    Genotype and index
    家系1
    Family 1
    家系2
    Family 2
    家系3
    Family 3
    XX/尾 雌性 Female 12.00±2.52a 9.33±2.91a 14.67±1.45a
    雄性 Male 1.00±0.58a 0.67±0.33a 0.33±0.33a
    XY/尾 雌性 Female 1.33±0.33a 1.33±0.33a 2.00±1.00a
    雄性 Male 29.33±2.33a 33.33±3.18a 33.33±5.70a
    YY/尾 雌性 Female 0.00±0.00a 0.00±0.00a 0.00±0.00a
    雄性 Male 7.67±0.67a 6.33±0.67a 6.33±0.67a
    成活率 Survival rate/% 51.33±5.04a 51.00±5.51a 56.67±8.09a
    XX雌性率 XX Female rate/% 90.91±5.25a 94.75±2.72a 97.44±2.56a
    XY雄性率 XY Male rate/% 95.74±0.75a 95.96±1.32a 94.56±0.79a
    YY雄性率 YY Male rate/% 100.00±0.00a 100.00±0.00a 100.00±0.00a
    下载: 导出CSV

    表  3  3个家系诱导组的成活率及性逆转率统计

    Table  3.   Statistics of survival rate and sex reversal rate of three induced families

    基因型及指标
    Genotype and index
    家系1
    Family 1
    家系2
    Family 2
    家系3
    Family 3
    雌性 Female/尾 29.50±7.50a 51.00±2.00a 38.50±7.50a
    雄性 Male/尾 1.00±1.00a 2.50±0.50a 1.50±0.50a
    XX/尾 6.50±0.50a 12.50±0.50b 8.00±0.00a
    XY/尾 17.00±5.00 a 27.50±1.50 a 28.50±5.50 a
    YY/尾 7.00±3.00a 13.50±0.50a 3.50±2.50a
    成活率
    Survival rate/%
    20.34±5.67a 35.67±1.00a 26.67±5.34a
    性逆转率
    Sex reversal rate/%
    97.00±3.00a 94.00±1.00a 95.55±0.50a
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-01-06
  • 修回日期:  2020-02-18
  • 录用日期:  2020-02-27
  • 网络出版日期:  2020-09-28
  • 刊出日期:  2020-06-05

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