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DNA条形码在篮子鱼科鱼类种类鉴定和系统进化分析中的应用

闫亚利 张楠 郭华阳 郭梁 朱克诚 刘宝锁 张殿昌

闫亚利, 张楠, 郭华阳, 郭梁, 朱克诚, 刘宝锁, 张殿昌. DNA条形码在篮子鱼科鱼类种类鉴定和系统进化分析中的应用[J]. 南方水产科学, 2019, 15(1): 100-105. doi: 10.12131/20180083
引用本文: 闫亚利, 张楠, 郭华阳, 郭梁, 朱克诚, 刘宝锁, 张殿昌. DNA条形码在篮子鱼科鱼类种类鉴定和系统进化分析中的应用[J]. 南方水产科学, 2019, 15(1): 100-105. doi: 10.12131/20180083
Yali YAN, Nan ZHANG, Huayang GUO, Liang GUO, Kecheng ZHU, Baosuo LIU, Dianchang ZHANG. Species identification and phylogenetic relationship in Siganidae based on DNA barcoding[J]. South China Fisheries Science, 2019, 15(1): 100-105. doi: 10.12131/20180083
Citation: Yali YAN, Nan ZHANG, Huayang GUO, Liang GUO, Kecheng ZHU, Baosuo LIU, Dianchang ZHANG. Species identification and phylogenetic relationship in Siganidae based on DNA barcoding[J]. South China Fisheries Science, 2019, 15(1): 100-105. doi: 10.12131/20180083

DNA条形码在篮子鱼科鱼类种类鉴定和系统进化分析中的应用

doi: 10.12131/20180083
基金项目: 中国水产科学研究院基本科研业务费专项资金 (2014A11);农业农村部财政专项(NFZX2013);国家科技基础性工作专项 (2013FY110700)
详细信息
    作者简介:

    闫亚利(1992—),女,硕士研究生,从事水产生物技术研究。E-mail: 2541733416@qq.com

    通讯作者:

    张殿昌(1977 — ),男,博士,研究员,从事水产种质资源与遗传育种研究。E-mail: zhangdch@scsfri.ac.cn

  • 中图分类号: Q 959

Species identification and phylogenetic relationship in Siganidae based on DNA barcoding

  • 摘要: 为建立篮子鱼科鱼类物种快速鉴定方法,该研究扩增获得了篮子鱼科10种鱼类的46条线粒体COⅠ基因序列,结合从BOLD和GenBank上筛选出的12种27条篮子鱼科COⅠ基因序列,分析篮子鱼科鱼类的COⅠ基因片段序列特征、种间和种内遗传距离及分子系统进化关系。结果表明,19种73条篮子鱼科COⅠ基因序列的平均碱基组成为T:28.9%、C:28.7%、A:24.6%、G:17.8%。G+C的含量(46.5%)低于A+T的含量(53.5%),碱基组成表现出明显的偏倚性;篮子鱼科鱼类的种间平均遗传距离为0.098,是种内平均遗传距离(0.002)的49倍;基于19种篮子鱼科鱼类的COⅠ序列的系统进化分析结果显示,19种篮子鱼中仅单斑篮子鱼(Siganus unimaculatus)和狐篮子鱼(S. vulpinus)聚类在一起,形成1个分支,剩余17种(89.5%)篮子鱼相同种内个体各自聚为一支,形成17个独立的分支。结果表明基于COⅠ基因的DNA条形码技术可应用于篮子鱼科物种鉴定。
  • 图  1  NJ 法构建的篮子鱼科鱼类系统进化树

    节点上数字显示1 000次重复的bootstrap验证分析中大于50%的支持率;标尺代表遗传距离单位为0.02·百万年–1

    Figure  1.  NJ tree resulting from analysis of COⅠ gene for 19 Siganidae species

    Values higher than 50% bootstrap in 1 000 replications are shown on branches. The scale represents the genetic distance of 0.02 per million years.

    图  2  长鳍篮子鱼 (全长179 mm) (a) 和褐篮子鱼 (全长267 mm) (b)

    Figure  2.  S. canaliculatus (total length=179 mm) (a) and S. fuscessens (total length=267 mm) (b)

    图  3  单斑篮子鱼 (全长162 mm) (a) 和狐篮子鱼 (全长121 mm) (b)

    Figure  3.  S. unimaculatus (total length=162 mm) (a) and S. vulpinus (total length=121 mm) (b)

    表  1  本研究篮子鱼科19种鱼类COⅠ基本信息

    Table  1.   Information of COⅠ genes from 19 Siganidae species

    序号
    No.
    物种
    species
    拉丁名
    Latin name
    样品数目
    number of samples
    Genbank序列号
    GenBank accession No.
    Gp1 银篮子鱼 Siganus argenteus 2 JQ350365, JQ350364
    Gp2 长鳍篮子鱼 Siganus canaliculatus 12 MH469588~MH469599
    Gp3 凹吻篮子鱼 Siganus corallinus 2 KP194212, KP194871
    Gp4 大瓮篮子鱼 Siganus doliatus 3 KP194691, KF930440, RESIC044-11
    Gp5 褐篮子鱼 Siganus fuscescens 3 KY372164, MH469600, MH469601
    Gp6 星斑篮子鱼 Siganus guttatus 6 MH469574~MH469579
    Gp7 爪哇篮子鱼 Siganus javus 2 KU692888, EU752210
    Gp8 截尾篮子鱼 Siganus luridus 3 BIM342-13, BIM364-13, MF409629
    Gp9 眼带篮子鱼 Siganus puellus 3 FJ584108, MH469572, MH469573
    Gp10 暗体篮子鱼 Siganus punctatissimus 1 KP194669
    Gp11 斑篮子鱼 Siganus punctatus 2 MH469570, MH469571
    Gp12 金带篮子鱼 Siganus rivulatus 3 KM538561, KM538560, BIM363-13
    Gp13 刺篮子鱼 Siganus spinus 5 KJ968266, KP193962, KF930443, MH469602, MH469603
    Gp14 白点篮子鱼 Siganus sutor 3 MF409513, MF409582, MF409508
    Gp15 单斑篮子鱼 Siganus unimaculatus 3 MH469558, MH469560, MH469561
    Gp16 蓝带篮子鱼 Siganus virgatus 8 MH469580~MH469587
    Gp17 狐篮子鱼 Siganus vulpinus 5 MH469562~MH469565, MH469557
    Gp18 蠕纹篮子鱼 Siganus vermiculatus 3 KF715016.1, KF715017.1, KF715018.1
    Gp19 大篮子鱼 Siganus magnificus 4 MH469566~MH469569
     注:本研究基因序列为MH469557~MH469558、MH469560~MH469603  Note: The GenBank accession No. are MH469557−MH469558 and MH469560−MH469603 in this study.
    下载: 导出CSV

    表  2  种间/种内遗传距离小于“10×”的物种对

    Table  2.   Species of interspecific/intraspecific distance ratio less than “10×”

    物种1 (种内遗传距离)
    Species 1 (intraspecific distance)
    物种2 (种内遗传距离)
    Species 2 (intraspecific distance)
    种间距离
    interspecific distance
    种间/种内遗传距离比
    intraspecific/intraspecific distance
    凹吻篮子鱼 S. corallinus (0.000) 大瓮篮子鱼 S. doliatus (0.004) 0.016 4.00
    大瓮篮子鱼 S. doliatus (0.004) 蓝带篮子鱼 S. virgatus (0.001) 0.008 2.00
    单斑篮子鱼 S. unimaculatus (0.002) 狐篮子鱼 S. vulpinus (0.003) 0.002 0.67
    单斑篮子鱼 S. unimaculatus (0.002) 大篮子鱼 S. magnificus (0.000) 0.010 5.00
    狐篮子鱼 S. vulpinus (0.003) 大篮子鱼 S. magnificus (0.000) 0.010 3.33
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-05-03
  • 修回日期:  2018-07-22
  • 网络出版日期:  2018-12-05
  • 刊出日期:  2019-02-05

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