闫亚利, 张楠, 郭华阳, 郭梁, 朱克诚, 刘宝锁, 张殿昌. DNA条形码在篮子鱼科鱼类种类鉴定和系统进化分析中的应用[J]. 南方水产科学, 2019, 15(1): 100-105. DOI: 10.12131/20180083
引用本文: 闫亚利, 张楠, 郭华阳, 郭梁, 朱克诚, 刘宝锁, 张殿昌. DNA条形码在篮子鱼科鱼类种类鉴定和系统进化分析中的应用[J]. 南方水产科学, 2019, 15(1): 100-105. DOI: 10.12131/20180083
YAN Yali, ZHANG Nan, GUO Huayang, GUO Liang, ZHU Kecheng, LIU Baosuo, ZHANG Dianchang. Species identification and phylogenetic relationship in Siganidae based on DNA barcoding[J]. South China Fisheries Science, 2019, 15(1): 100-105. DOI: 10.12131/20180083
Citation: YAN Yali, ZHANG Nan, GUO Huayang, GUO Liang, ZHU Kecheng, LIU Baosuo, ZHANG Dianchang. Species identification and phylogenetic relationship in Siganidae based on DNA barcoding[J]. South China Fisheries Science, 2019, 15(1): 100-105. DOI: 10.12131/20180083

DNA条形码在篮子鱼科鱼类种类鉴定和系统进化分析中的应用

Species identification and phylogenetic relationship in Siganidae based on DNA barcoding

  • 摘要: 为建立篮子鱼科鱼类物种快速鉴定方法,该研究扩增获得了篮子鱼科10种鱼类的46条线粒体COⅠ基因序列,结合从BOLD和GenBank上筛选出的12种27条篮子鱼科COⅠ基因序列,分析篮子鱼科鱼类的COⅠ基因片段序列特征、种间和种内遗传距离及分子系统进化关系。结果表明,19种73条篮子鱼科COⅠ基因序列的平均碱基组成为T:28.9%、C:28.7%、A:24.6%、G:17.8%。G+C的含量(46.5%)低于A+T的含量(53.5%),碱基组成表现出明显的偏倚性;篮子鱼科鱼类的种间平均遗传距离为0.098,是种内平均遗传距离(0.002)的49倍;基于19种篮子鱼科鱼类的COⅠ序列的系统进化分析结果显示,19种篮子鱼中仅单斑篮子鱼(Siganus unimaculatus)和狐篮子鱼(S. vulpinus)聚类在一起,形成1个分支,剩余17种(89.5%)篮子鱼相同种内个体各自聚为一支,形成17个独立的分支。结果表明基于COⅠ基因的DNA条形码技术可应用于篮子鱼科物种鉴定。

     

    Abstract: In order to establish a rapid and convenient method for fish species identification of Siganidae, we obtained 46 COⅠ genes from 10 species and downloaded 27 COⅠ genes from 12 species from BOLD and GenBank to study the COⅠ gene's genetic composition, intraspecific and interspecific genetic distance, codon characteristics and molecular phylogenetic tree. The results show that the average base composition of 73 COⅠ genes was T: 28.9%, C: 28.7%, A: 24.6% and G: 17.8%. The content of G+C (46.5%) was lower than the content of A+T (53.5%) and the base composition showed obvious bias. The average interspecific genetic distance was 0.098, which was 49 times the length of the genetic distance within the species (average: 0.002). The phylogenetic tree reveals that Siganus unimaculatus and S. vulpinus clustered into one branch among 19 species of basket fish. The other 17 species (89.5%) clustered in monophyletic groups at genus level. It is shown that the technology of DNA barcoding can be used for fish species identification of Siganidae.

     

/

返回文章
返回