Cloning of GLUT1 gene fromwinged pearl oyster Pteria penguin and its expression in response to glucose challenge

Facilitated glucose transporter type 1 (GLUT1) takes part in transporting glucose in most animal tissues responsible for energy metabolism. To study function of GLUT1 in Pteria penguin, the cDNA sequence of GLUT1 was obtained from P.penguin transcriptome using RACE, which was named as PpGLUT1. The full length cDNA was 2 068 bp, The gene encoded 522 amino acids, 12 transmembrane helical motifs and multiple phosphorylation sites, with N-terminal and C-terminal on the cytoplasmic side, molecular weight of 57.226 4 kD, and isoelectric point of 5.17. Fluorescent quantitative PCR results show that PpGLUT1 expressed in all tissues and significantly low in muscle. The high concentration of 0.5 gmL-1 glucose injection result shows that the expression of PpGLUT1 increased within 30 min after injected, and then decreased; at the injection time of 2 h, the expression of PpGLUT1 was lowest; the expression gradually increased after 2 h. Different concentrations of glucose injection test indicate that the expression levels of PpGLUT1 increased with increase of glucose concentrations ranging from 0.1 gmL-1 to 0.4 gmL-1 and then decreased. The mRNA expression responses to glucose suggests that PpGLUT1 play an important role in glucose transportation in multiple tissues of P. penguin.