Characterization and expression analysis of GPP94 in black tiger shrimp (Penaeus monodon) under different acute stresses

In the present study, the full-length of PmGRP94 was cloned from Penaeus monodon using RACE-PCR. The full-length of PmGRP94 cDNA was 2 990 bp containing a 5untranslated region (UTR) of 75 bp, a 3UTR of 527 bp and an open reading frame (ORF) of 2 388 bp. We analyzed the characterization and expression of tissue specificity of PmGRP94 when the shrimps were exposed with different pH values, salinities and three heavy metals using real time PCR (RT-PCR). The highest expression level was found in hepatopancreas. The transcription levels of PmGRP94 were significantly up-regulated under pH, copper and znic challenges. The results suggest that PmGRP94 might be a promising candidate marker for pH stress, cadmium and manganese exposure in shrimps.