LOU Zhongwei, XU Kunhua, WANG Xiao, RAO Huajun, DAI Zhiyuan. Characteristics of transglutaminase from bighead carp[J]. South China Fisheries Science, 2014, 10(6): 72-77. DOI: 10.3969/j.issn.2095-0780.2014.06.010
Citation: LOU Zhongwei, XU Kunhua, WANG Xiao, RAO Huajun, DAI Zhiyuan. Characteristics of transglutaminase from bighead carp[J]. South China Fisheries Science, 2014, 10(6): 72-77. DOI: 10.3969/j.issn.2095-0780.2014.06.010
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Characteristics of transglutaminase from bighead carp

    Abstract
  • We studied the transglutaminase (TGase) from bighead carp (Hypophthalmichthys nobilis) under different conditions (temperature, pH, DTT). The optimum temperature and pH of TGase were 37~45 ℃ and 6.5~7.5, respectively. The optimum concentrations of CaCl2 and dithiothreitol were 0~2.2 mmolL-1 and 0~0.2mmolL-1, respectively. The TGase activity decreased as concentrations of Na+ and K+ increased, and was inactivated by Mg2+, Cu2+，Zn2+, Ba2+，Fe3+，Fe2+，Pb2+ and EDTA.
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