ZHU Lijing, SUN Zhongxiang, CHEN Shuyin, JI Hongjiu, XU Xiaofeng, SHEN Songdong. Microsatellite enrichment by magnetic beads in Coelomactra antiquate[J]. South China Fisheries Science, 2011, 7(4): 10-15. DOI: 10.3969/j.issn.2095-0780.2011.04.002
Citation: ZHU Lijing, SUN Zhongxiang, CHEN Shuyin, JI Hongjiu, XU Xiaofeng, SHEN Songdong. Microsatellite enrichment by magnetic beads in Coelomactra antiquate[J]. South China Fisheries Science, 2011, 7(4): 10-15. DOI: 10.3969/j.issn.2095-0780.2011.04.002
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Microsatellite enrichment by magnetic beads in Coelomactra antiquate

    Abstract
  • In this experiment, magnetic bead hybridization method was used to enrich the microsatellite loci in Coelomactra antiquate. As a result, 38 microsatellites that repeated no less than 5 times were found. Among the 38 microsatellites, 68.4% are perfect, 23.7% are imperfect and the rest are compound (7.9%). In addition to the biotin-labeled SRS (simple repetitive sequence) probe CA and GA, there are ATTG, TG, CGTG and TGTTG in the microsatellites. Microsatellite repeats mainly from 5 to 46 times, and the most repetitive number is 70. We designed 14 pairs of SSR-primers using these microsatellite sites, which will facilitate the studies on the genetic diversity of C.antiquata.
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