Analysis of volatile components and trace elements in flesh of Pinctada martensii with black shell disease

The flesh of Pinctada martensii with black shell disease was processed by adopting methods such as wet digestion and Soxhlet extraction + steam distillation. The main 12 trace elements of the samples were determined by ICP-AES and the volatile components were identified by GC-MS. Compared with healthy shellfish, there was no significant difference in the content of trace elements except for Zn and Se (decrease rates were 8.54% and 10.98% respectively) in sick P.martensii. The sick shellfish contained not only some metal elements and a large quantity of benzene ring, but also 16 kinds of volatile components while healthy shellfish had 20.