HE Junyan, LI Laihao, HAO Shuxian, YANG Xianqing, MA Haixia, CEN Jianwei, Shi Hong, HU Qiangchu. Separation of myoglobin from tilapia by ammonium sulfate salting-out[J]. South China Fisheries Science, 2009, 5(2): 17-22. DOI: 10.3969/j.issn.1673-2227.2009.02.003
Citation: HE Junyan, LI Laihao, HAO Shuxian, YANG Xianqing, MA Haixia, CEN Jianwei, Shi Hong, HU Qiangchu. Separation of myoglobin from tilapia by ammonium sulfate salting-out[J]. South China Fisheries Science, 2009, 5(2): 17-22. DOI: 10.3969/j.issn.1673-2227.2009.02.003

Separation of myoglobin from tilapia by ammonium sulfate salting-out

  • Effect of ammonium sulfate on salting-out of myoglobin was studied by analysing color, Mb content, Mb purity, recovery rate and absorption spectra. The results showed that the myoglobin could be precipitated after treating with ammonium sulfate at saturation 60%~100%, and the purity and recovery rate were 37.52% and 39.89% respectively after treated with 50% ammonium sulfate. Mb purity was slightly decreased but the recovery rate was increased gradually when the saturation was greater than 60%. And recovery rate was up to 87.56% when the saturation reached to 100%. Oxymyoglobin was partly oxidated into the met-myoglobin in the process of the ammonium sulfate salting-out.
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