Optimization of multi-enzymatic extraction of polysaccharide from Gelidium amansii by response surface methodology

To optimize the multi-enzymatic extraction process of polysaccharides from Gelidium amansii, we designed a three-factor and three-level Box-Benhnken center-united experiment based on single factor test, and conducted a regression analysis. The optimum technological parameters of enzymatic extraction of G. amansii polysaccharide were: enzymes addition 2.30%, enzymatic hydrolysis temperature 60.43 ℃, ratio of material to water 1∶32 (g·mL⁻¹). The extraction yield of polysaccharide was up to 15.98% after 120 min of enzymatic hydrolysis under these conditions.