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硫酸铜治疗卵形鲳鲹淀粉卵涡鞭虫病的研究

李志成 江飚 钟志鸿 李诗钰 何润真 唐嘉嘉 李安兴

李志成, 江飚, 钟志鸿, 李诗钰, 何润真, 唐嘉嘉, 李安兴. 硫酸铜治疗卵形鲳鲹淀粉卵涡鞭虫病的研究[J]. 南方水产科学. doi: 10.12131/20200260
引用本文: 李志成, 江飚, 钟志鸿, 李诗钰, 何润真, 唐嘉嘉, 李安兴. 硫酸铜治疗卵形鲳鲹淀粉卵涡鞭虫病的研究[J]. 南方水产科学. doi: 10.12131/20200260
Zhicheng LI, Biao JIANG, Zhihong ZHONG, Shiyu LI, Runzhen HE, Jiajia TANG, Anxing LI. Treatment of amyloodiniosis in golden pompano (Trachinotus ovatus) by copper sulfate[J]. South China Fisheries Science. doi: 10.12131/20200260
Citation: Zhicheng LI, Biao JIANG, Zhihong ZHONG, Shiyu LI, Runzhen HE, Jiajia TANG, Anxing LI. Treatment of amyloodiniosis in golden pompano (Trachinotus ovatus) by copper sulfate[J]. South China Fisheries Science. doi: 10.12131/20200260

硫酸铜治疗卵形鲳鲹淀粉卵涡鞭虫病的研究

doi: 10.12131/20200260
基金项目: 现代农业产业体系海水鱼寄生虫病防控岗位项目资助 (CARS-47-G18)
详细信息
    作者简介:

    李志成 (1995—),男,硕士研究生,研究方向为海水鱼类寄生虫病防控。E-mail: 839387965@qq.com

    通讯作者:

    李安兴 (1963—),男,博士,教授,从事水生经济动物寄生生物病害控制研究。E-mail: lianxing@mail.sysu.edu.cn

  • 中图分类号: S 942.2+2

Treatment of amyloodiniosis in golden pompano (Trachinotus ovatus) by copper sulfate

  • 摘要: 为更科学地使用硫酸铜治疗淀粉卵涡鞭虫病,该研究以卵形鲳鲹 (Trachinotus ovatus) 为动物模型,探讨了硫酸铜对眼点淀粉卵涡鞭虫 (Amyloodinium ocellatum) 生活史各个阶段的有效驱杀浓度和作用时间,并评估其对卵形鲳鲹幼鱼的安全质量浓度范围。结果显示,卵形鲳鲹幼鱼对硫酸铜的耐受性强,安全质量浓度小于43.06 mg·L−1。用3.13、0.78、0.20 mg·L−1硫酸铜溶液分别药浴处理10、30、60 min可100%驱杀涡孢子;用2.0、1.0、0.5 mg·L−1硫酸铜溶液分别药浴浸泡鱼体2、4、8 h可100%清除鱼体上的营养体;而包囊对硫酸铜的耐受性强,用100 mg·L−1硫酸铜溶液连续药浴,仍有90%以上的包囊能继续分裂。治疗实验显示,在0.2、0.4 mg·L−1硫酸铜溶液中连续药浴10 d,对患病鱼的相对保护率分别为80%和90%,表明使用低浓度硫酸铜溶液连续药浴可有效治疗卵形鲳鲹淀粉卵涡鞭虫病。
  • 图  1  寄生在病鱼鳃处的眼点淀粉卵涡鞭虫营养体显微镜观察

    a. 感染鱼鳃上营养体寄生情况;b. 感染鱼经1 mg·L−1硫酸铜溶液浸泡1 h后鳃上营养体寄生情况;c. 感染鱼经1 mg·L−1硫酸铜溶液浸泡4 h后鳃上营养体寄生情况

    Figure  1.  Microscopic observation of trophonts on gill of infection fish

    a. Parasitism of trophonts on the gill of infected fish; b. Parasitism of trophonts on the gills of infected fish after being immersed in 1 mg·L−1 copper sulfate solution for 1 h; c. Parasitism of trophonts on the gills of infected fish after being immersed in 1 mg·L−1 copper sulfate solution for 4 h

    图  2  不同处理组卵形鲳鲹的死亡率

    Figure  2.  Mortality of T. ovatus in different groups

    表  1  硫酸铜溶液对眼点淀粉卵涡鞭虫涡孢子的杀灭效果

    Table  1.   Effect of copper sulfate solution on A. ocellatum dinospores

    时间
    t/min
    硫酸铜质量浓度 Concentration of copper sulfate/(mg·L−1)
    100502512.56.253.131.560.780.390.200.100.050.0250
    10+++++++++++++++++++++++++++++++++
    30+++++++++++++++++++++++++++++++++++++++++
    60++++++++++++++++++++++++++++++++++++++++++++
    120++++++++++++++++++++++++++++++++++++++++++++
    注:“++++”、“+++”、“++”、“+”和“−”分别代表能引起100%、75%、50%、25%和0%涡孢子死亡 Note: "++++", "+++", "++", "+" and "−" represent the mortalities of dinospores were 100%, 75%, 50%, 25% and 0%, respectively.
    下载: 导出CSV

    表  2  硫酸铜溶液对眼点淀粉卵涡鞭虫包囊分裂率的影响

    Table  2.   Effect of copper sulfate solution on A. ocellatum tomonts cleavage rate %

    硫酸铜质量浓度
    Concentration of copper sulfate/(mg·L−1)
    浸泡时间 Immersing time/h
    24487296
    100.0090.92±1.90ab90.92±1.90ab90.92±1.90ab90.92±1.90ab
    50.0093.95±1.93ab93.95±1.93ab93.95±1.93ab93.95±1.93ab
    25.0090.50±2.60b90.50±2.60b90.50±2.60b90.50±2.60b
    12.5094.88±0.52 ab94.88±0.52ab94.88±0.52ab94.88±0.52ab
    6.2595.33±1.16 ab95.33±1.16ab95.33±1.16ab95.33±1.16ab
    3.1397.10±2.52ab97.10±2.52ab97.10±2.52ab97.10±2.52ab
    1.5697.13±2.66ab97.13±2.66ab97.13±2.66ab97.13±2.66ab
    0.7897.33±2.329a97.33±2.329a97.33±2.329a97.33±2.329a
    0.3996.91±2.75ab96.91±2.75ab96.91±2.75ab96.91±2.75ab
    0.2096.10±3.62ab96.10±3.62ab96.10±3.62ab96.10±3.62ab
    0.1095.55±0.51ab95.55±0.51ab95.55±0.51ab95.55±0.51ab
    096.94±2.68ab96.94±2.68ab96.94±2.68ab96.94±2.68ab
    注:同行数据不同字母表示差异显著 (P<0.05) Note: Values with different superscripts in the same row are significantly different (P<0.05).
    下载: 导出CSV

    表  3  卵形鲳鲹幼鱼对硫酸铜溶液的耐受力

    Table  3.   Tolerance of juvenile T. ovatus to copper sulfate solution

    时间
    t/h
    硫酸铜质量浓度
    Concentration of copper sulfate/(mg·L−1)
    300230.77177.51136.55105.040
    24 100 55 35 10 5 0
    48 100 75 50 15 10 0
    72 100 85 55 30 10 0
    96 100 85 55 30 10 0
    下载: 导出CSV

    表  4  不同质量浓度硫酸铜浸泡不同时间的相对驱虫率

    Table  4.   Relative deworming rate of different mass concentrations of copper sulfate immerse for different time

    硫酸铜质量浓度
    Concentration of copper sulfate/(mg·L−1)
    浸泡时间 Immersing time/h
    0.51248
    4.0 98.29±1.49 98.69±0.76 100.00±0.00
    2.0 95.85±2.41 98.24±1.56 100.00±0.00
    1.0 66.00±8.59 88.82±3.75 96.73±2.03 100.00±0.00
    0.5 43.72±14.77 75.25±12.26 90.95±4.04 97.99±2.05 100.00±0.00
    0.4 9.96±2.59 33.94±0.29 85.09±3.69 95.80±1.24 99.46±0.23
    0.3 14.43±1.44 13.82±1.72 27.37±1.31 27.64±2.26 64.02±2.59
    0.2 7.11±7.19 16.26±14.95 14.84±0.86 21.27±3.78 66.26±3.66
    0.0 1.16±6.54 2.61±2.72 −3.04±4.58 0.14±5.31 2.17±5.43
    注:−. 未取样 Note: −. Not sampled
    下载: 导出CSV
  • PAPERNA I. Amyloodinium ocellatum (Brown, 1931) (Dinoflagellida) infestations in cultured marine fish at Eilat, Red Sea: epizootiology and pathology[J]. J Fish Dis, 1980, 3(5): 363-372. doi: 10.1111/j.1365-2761.1980.tb00421.x
    MOREIRA M, CORDEIRO-SILVA A, BARATA M, et al. Influence of age on stress responses of white seabream to amyloodiniosis[J]. Fishes, 2019, 4(2): 26. doi: 10.3390/fishes4020026
    NOGA E J. Propagation in cell culture of the dinoflagellate amyloodinium, an ectoparasite of marine fishes[J]. Science, 1987, 236(4806): 1302-1304. doi: 10.1126/science.236.4806.1302
    WOO P T K, ARDELLI B F. Immunity against selected piscine flagellates[J]. Dev Comp Immunol, 2014, 43(2): 268-279. doi: 10.1016/j.dci.2013.07.006
    GÓMEZ F, GAST R J. Dinoflagellates amyloodinium and ichthyodinium (Dinophyceae), parasites of marine fishes in the South Atlantic Ocean.[J]. Dis Aquat Organ, 2018, 131(1): 29-37. doi: 10.3354/dao03274
    MARTINS M L, CARDOSO L, MARCHIORI N, et al. Protozoan infections in farmed fish from Brazil: diagnosis and pathogenesis[J]. Rev Bras Parasitol V, 2015, 24(1): 1-20. doi: 10.1590/S1984-29612015013
    KUMAR P R, NAZAR A K A, JAYAKUMAR R, et al. Amyloodinium ocellatum infestation in the broodstock of silver pompano Trachinotus blochii (Lacepede, 1801) and its therapeutic control[J]. Ind J Fish, 2015, 62(1): 131-134.
    BROWN E M. Note on a new species of Dinoflagellate from the gills and epidermis of marine fishes[J]. J Zool, 1931, 101(1): 345-346.
    MOREIRA C B, HASHIMOTO G S O, ROMBENSO A N, et al. Outbreak of mortality among cage-reared cobia (Rachycentron canadum) associated with parasitism[J]. Rev Bras Parasitol V, 2013, 22(4): 588-591. doi: 10.1590/S1984-29612013000400021
    BAHRI S. Protozoan and myxozoan infections in wild gilthead seabream (Sparus aurata L.) from North Lake of Tunis, Tunisia[J]. Acta Parasitol, 2012, 57(2): 114-121.
    黄倩倩, 林黑着, 周传朋, 等. 卵形鲳鲹幼鱼对维生素B2的需要量[J]. 南方水产科学, 2019, 15(1): 69-76.
    李秀玲, 刘宝锁, 张楠, 等. 发酵豆粕替代鱼粉对卵形鲳鲹生长和血清生化的影响[J]. 南方水产科学, 2019, 15(4): 68-75.
    孙莘溢, 黄小林, 黄忠, 等. 卵形鲳鲹摄食、耗氧节律和胃肠排空时间的研究[J]. 南方水产科学, 2019, 15(5): 77-83.
    韦明利, 周胜杰, 陈旭, 等. 北海市铁山港卵形鲳鲹深水网箱小瓜虫暴发简报[J]. 科学养鱼, 2020(2): 45-46.
    QIAO Y, SHAO Y X, PENGSAKUL T, et al. Morphological and molecular characterization of Ceratomyxa batam n. sp. (Myxozoa: Ceratomyxidae) infecting the gallbladder of the cultured Trachinotus ovatus (Perciformes: Carangidae) in Batam Island, Indonesia[J]. Parasitol Res, 2019, 118: 1647-1651. doi: 10.1007/s00436-019-06217-w
    涂志刚, 崔婧, 严耿杰, 等. 网箱养殖卵形鲳鲹“烂身病”病原分离鉴定及药敏分析[J]. 水产科学, 2018, 37(5): 634-639.
    许海东, 区又君, 郭志勋, 等. 神经坏死病毒对卵形鲳鲹的致病性及外壳蛋白基因序列分析[J]. 上海海洋大学学报, 2010, 19(4): 482-488.
    熊向英, 徐力文, 董兰芳, 等. 网箱养殖卵形鲳鲹鱼体寄生虫初步调查[J]. 广西科学院学报, 2015, 31(4): 281-285.
    BESSAT M, FADEL A. Amyloodiniosis in cultured Dicentrarchus labrax: parasitological and molecular diagnosis, and an improved treatment protocol[J]. Dis Aquat Organ, 2018, 129(1): 41-51. doi: 10.3354/dao03237
    何祥楷. 大黄鱼苗淀粉卵涡鞭虫病的诊断及防治方法[J]. 福建水产, 2013, 35(6): 475-479.
    徐绍刚, 朱华, 田照辉, 等. 漠斑牙鲆淀粉卵甲藻病的防治[J]. 科学养鱼, 2008(7): 48-49.
    VIRGULA J C, CRUZ-LACIERDA E R, ESTANTE E G, et al. Copper sulfate as treatment for the ectoparasite Amyloodinium ocellatum (Dinoflagellida) on milkfish (Chanos chanos) fry[J]. Aacl Bioflux, 2017, 10(2): 365-371.
    VAZ C, AFONSO F, BARATA M, et al. Effect of copper exposure and recovery period in reared Diplodus sargus[J]. Ecotoxicology, 2019, 28(9): 1075-1084. doi: 10.1007/s10646-019-02109-y
    BOWER C E, BIEVER T R C. A standardized method of propagating the marine fish parasite, Amyloodinium ocellatum[J]. J Parasitol, 1987, 73(1): 85-88. doi: 10.2307/3282348
    江飚, 王晶, 罗恒利, 等. 福尔马林防治大黄鱼刺激隐核虫病的研究[J]. 水产科学, 2018, 37(3): 336-341.
    STRAUS D L, MITCHELL A J, CARTER R R, et al. Hatch rate of channel catfish Ictalurus punctatus (Rafinesque 1818) eggs treated with 100 mg/L copper sulphate pentahydrate[J]. Aquacult Res, 2012, 43(1): 14-18. doi: 10.1111/j.1365-2109.2010.02791.x
    王秋实, 王乐, 裴月, 等. 硫酸铜对梭鲈幼鱼的急性毒性及其对车轮虫的灭杀作用[J]. 水产学杂志, 2018, 31(1): 17-20.
    PAPERNA I. Chemical control of Amyloodinium ocellatum (Brown 1931) (Dinoflagellida) infections: In vitro tests and treatment trials with infected fishes[J]. Aquaculture, 1984, 38(1): 1-18. doi: 10.1016/0044-8486(84)90133-9
    黄殿盛. 基于核糖体序列的眼点淀粉卵甲藻和刺激隐核虫的分类学分析与检测[D]. 福州: 福建农林大学, 2016: 9-12.
    EWA L T, DAWID G, EWA D K, et al. Changes in hematological parameters, and copper and iron concentrations in tissues of prussian carp during depuration period after the previous exposure to copper in water[J]. Turk J Fish Aquat Sci, 2018, 19(9): 753-763.
    MONTEIRO S M, OLIVEIRA E, FONTAÍNHAS-FERNANDES A A, et al. Effects of sublethal and lethal copper concentrations on the gill epithelium ultrastructure of Nile tilapia, Oreochromis niloticus[J]. Zool Stud, 2012, 51(7): 977-987.
    STRAUS D L. Species sensitivity to copper[J]. J Appl Aquacult, 2006, 18(1): 89-99. doi: 10.1300/J028v18n01_06
    CHAKOUMAKOS C, RUSSO R C, THURSTON R V, et al. Toxicity of copper to cutthroat trout (Salmo clarki) under different conditions of alkalinity, pH and hardness[J]. Amer Chem Soc, 1979, 13: 213-219.
    MILLER T G, MACKAY W C. The effects of hardness, alkalinity and pH of test water on the toxicity of copper to rainbow trout (Salmo gairdneri)[J]. Water Res, 1980, 14: 129-133. doi: 10.1016/0043-1354(80)90228-6
    程敏红, 杨小立, 庞强, 等. 5种常用渔药对斜带石斑鱼幼鱼的急性毒性试验[J]. 水产科学, 2014, 33(2): 69-74.
    范超, 史成银. 硫酸铜对斑石鲷幼鱼的急性毒性及对眼点淀粉卵涡鞭虫的杀灭效果[J]. 中国动物检疫, 2016, 33(11): 98-102.
    LAWLER A R. Studies on Amyloodinium ocellatum (Dinoflagellata) in Mississippi Sound: natural and experimental hosts[J]. Gulf Res Rep, 1980, 6(4): 403-413.
    KUPERMAN B, MATEY V. Massive infestation by Amyloodinium ocellatum (Dinoflagellida) of fish in a highly saline lake, Salton Sea, California, USA[J]. Dis Aquat Organ, 1999, 39(1): 65-73.
    COLORNI A. Hyperparasitism of Amyloodinium ocellatum (Dinoflagellida: Oodinidae) on Neobenedenia melleni (Monogenea: Capsalidae)[J]. Dis Aquat Organ, 1994, 19(2): 157-159.
    范超. 斑石鲷卵鞭虫病和上皮囊肿病的研究[D]. 上海: 上海海洋大学, 2016: 3-6.
    PAPERNA I. Reproduction cycle and tolerance to temperature and salinity of Amyloodinium ocellatum (Brown, 1931) (Dinoflagellida)[J]. Ann Parasit Hum Comp, 1984, 59(1): 7-30. doi: 10.1051/parasite/1984591007
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  • 收稿日期:  2020-12-22
  • 修回日期:  2021-02-22
  • 网络出版日期:  2021-03-31

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