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牡蛎及其酶解产物抗皮肤光老化的初步研究

吕佳桐 林海生 秦小明 章超桦 曹文红 高加龙 郑慧娜

吕佳桐, 林海生, 秦小明, 章超桦, 曹文红, 高加龙, 郑慧娜. 牡蛎及其酶解产物抗皮肤光老化的初步研究[J]. 南方水产科学. doi: 10.12131/20200141
引用本文: 吕佳桐, 林海生, 秦小明, 章超桦, 曹文红, 高加龙, 郑慧娜. 牡蛎及其酶解产物抗皮肤光老化的初步研究[J]. 南方水产科学. doi: 10.12131/20200141
Jiatong LÜ, Haisheng LIN, Xiaoming QIN, Chaohua ZHANG, Wenhong CAO, Jialong GAO, Huina ZHENG. Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging[J]. South China Fisheries Science. doi: 10.12131/20200141
Citation: Jiatong LÜ, Haisheng LIN, Xiaoming QIN, Chaohua ZHANG, Wenhong CAO, Jialong GAO, Huina ZHENG. Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging[J]. South China Fisheries Science. doi: 10.12131/20200141

牡蛎及其酶解产物抗皮肤光老化的初步研究

doi: 10.12131/20200141
基金项目: 现代农业产业技术体系建设专项资金 (CARS-49);国家重点研发计划项目“水产品非宏量营养素的健康功效解析” (2018YFD0901105)
详细信息
    作者简介:

    吕佳桐 (1995—),女,硕士研究生,研究方向为海洋功能活性物质。E-mail: 1141432665@qq.com

    通讯作者:

    秦小明 (1964—),男,博士,教授,从事水产品精深加工、贝类活性物质研究。E-mail: xiaoming0502@21cn.scom

  • 中图分类号: TS 254.9

Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging

  • 摘要: 文章探讨了香港牡蛎 (Crassostrea hongkongensis) 肉粉 (Oyster meat, OM) 及其酶解产物 (Oyster hydrolysis, OH) 对紫外线诱导的小鼠 (Mus musculus) 皮肤光老化的保护作用。紫外线 (UVA+UVB) 每天照射构建小鼠皮肤光老化模型并灌胃OM和OH,分为低、中、高3组 (30、90、180 mg·kg−1) 给药,持续8周。结果表明,OM和OH可减轻小鼠皮肤皱纹,提高皮肤弹性,减少皮下血管的萎缩,防止血管通透性增加;皮肤HE染色结果表明,中高剂量的OM和OH可显著减缓表皮层过度角化增厚 (P<0.05);masson染色和醛品红染色结果表明,OM和OH可减少胶原纤维的卷曲和降解,避免弹性纤维的异常增生,恢复真皮细胞外基质网络的排列;与中剂量组相比,OM和OH可使皮肤中超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化氢酶 (GSH-PX) 的酶活力显著提高,同时丙二醛 (MDA) 和8-羟基脱氧鸟苷 (8-OHDG) 的浓度降低 (P<0.05);OM和OH可抑制基质金属蛋白酶 (MMP3、MMP9) 的表达,显著降低真皮中羟脯胺酸 (Hyp) 的降解;此外,高剂量的OH可显著降低皮肤中炎症因子细胞白介素6 (IL-6) 和肿瘤坏死因子 (TNF-α) 的表达,并通过提高转化生长因子 (TGF-β) 来提高皮肤抗炎能力。
  • 图  1  牡蛎及其酶解产物对小鼠光老化皮肤表征的影响

    a. 小鼠背部皮肤的宏观外观;b. 小鼠皮下血管状态;图1-b中黑色箭头指示血管通透性增加引发炎症,黄色圆圈区域指示血管萎缩

    Figure  1.  Effects of oyster and its enzymatic hydrolysis products on skin characterization of photoaging skin in mice

    a. Macroscopic appearance of skin on the back of mice; b. State of subcutaneous blood vessels in mice; the black arrows in Figure 1-b indicate inflammation caused by increased vascular permeability, and the yellow circle area indicates vascular atrophy.

    图  2  牡蛎及其酶解产物对小鼠光老化皮肤结构和表皮层厚度的影响

    同一指标中标有不同字母代表差异显著 (P<0.05);图4图6同此

    Figure  2.  Effect of oysters and their enzymatic hydrolysis products on the structure and thickness of photoaging skin in mice

    For the same indicator, different letters indicate significant difference (P<0.05). The same case in Figure 4-6.

    图  3  牡蛎及其酶解产物对小鼠光老化皮肤中胶原纤维 (a) 和弹力纤维 (b) 的影响 (×100)

    图3-a中黑色箭头指示混乱卷曲的胶原纤维,黄色箭头指示排列规律的胶原纤维;图3-b中黑色箭头指示弹力纤维在表皮层的异常增生

    Figure  3.  Effect of oysters and their enzymatic hydrolysis products on collagen fibers (a) and elastic fibers (b) in photoaging skin of mice (×100)

    In Figure 3-a, the black arrows indicate the disorderly and curly collagen fibers, and the yellow arrows indicate the regularly arranged collagen fibers; in Figure 3-b, the black arrows indicate the abnormal proliferation of elastic fibers in the epidermis.

    图  4  牡蛎及其酶解产物对超氧化物歧化酶、谷胱甘肽过氧化氢酶、丙二醛和8-羟基脱氧鸟苷的影响

    Figure  4.  Effect of oysters and their enzymatic hydrolysis products on SOD, GSH-PX, MDA and 8-OHDG

    图  5  牡蛎及其酶解产物对MMP3 (a)、MMP9 (b)和羟脯胺酸 (c) 的影响

    Figure  5.  Effect of oysters and their enzymatic hydrolysis products on MMP3 (a), MMP9 (b) and Hyp (c)

    图  6  牡蛎及其酶解产物对白介素6、肿瘤坏死因子α (b) 和转移生长因子β (c) 的影响

    Figure  6.  Effect of oysters and their enzymatic hydrolysis products on IL-6 (a), TNF-α (b) and TGF-β (c)

    表  1  分组、照射及灌胃剂量

    Table  1.   Grouping, irradiation and intragastric dosage

    组名    
    Group    
    是否照射UVA+UVB
    Whether to irradiate UVA+UVB
    每日灌胃剂量
    Daily intragastric dose/(mg·kg−1)
    空白组 Blank group (B)
    模型组 Model group (M)
    阳性对照组 Positive control group (P) 10
    全牡蛎低剂量组 Oyster meat low dose (OM-L) 30
    全牡蛎中剂量组 Oyster meat medium dose (OM-M) 90
    全牡蛎高剂量组 Oyster meat high dose (OM-H) 180
    牡蛎酶解低剂量组 Oyster hydrolysis low dose (OH-L) 30
    牡蛎酶解中剂量组 Oyster hydrolysis medium dose (OH-M) 90
    牡蛎酶解高剂量组 Oyster hydrolysis high dose (OH-H) 180
    注:每日光照后给药,维A酸的剂量以维A酸质量计,全牡蛎粉及牡蛎酶解粉的剂量以蛋白质量计 Note: After daily light administration, the dosage of retinoic acid is based on the quality of retinoic acid, and the dosages of whole oyster powder and oyster enzymolysis powder are based on protein quality.
    下载: 导出CSV

    表  2  皮肤皱纹等级评分标准

    Table  2.   Skin wrinkle rating scale

    评分
    Score
    评分标准
    Grading
    0 无褶皱或松弛,无增厚,纵向细条纹贯穿背部,随运动出现或消失
    1 皮肤大部分为纵向细纹,少部分横向细纹,可随运动出现或消失
    2 尚存在少量纵向细纹,大部分横向细纹,随运动出现或消失,皮肤稍有增厚
    3 纵向细纹全部消失,出现少量横向浅皱纹,不随运动出现或消失,皮肤稍有增厚
    4 持续存在横向深皱纹和松弛,皮肤泛红,皮肤明显增厚
    5 持续存在大量横向粗糙深皱纹和松弛,出现小红斑,皮肤明显增厚
    6 严重的皱纹,大面积红斑,肿瘤或病变的发展,皮肤增生异常
    下载: 导出CSV

    表  3  皮肤表征评价

    Table  3.   Evaluation of skin characterization $ \overline { X}{\bf \pm {SD}}$

    组别    
    Group     
    皮肤评分
    Skin score
    提皮恢复时间
    Skin recovery time/s
    皮肤总厚度
    Total skin thickness/mm
    空白组 Blank group (B) 0.00±0.00f 1.97±0.19e 0.39±0.03f
    模型组 Model group (M) 5.60±0.49a 8.40±1.29a 1.01±0.06a
    阳性对照组 Positive control group (P) 2.00±0.63de 2.54±1.00de 0.66±0.05e
    全牡蛎低剂量组 Oyster meat low dose (OM-L) 3.80±0.40b 4.99±0.68b 0.92±0.07b
    全牡蛎中剂量组 Oyster meat medium dose (OM-M) 3.20±0.40bc 4.31±0.27bc 0.83±0.05cd
    全牡蛎高剂量组 Oyster meat high dose (OM-H) 2.80±0.40c 2.30±0.38e 0.71±0.04e
    牡蛎酶解低剂量组 Oyster hydrolysis low dose (OH-L) 3.20±0.40bc 4.80±0.20b 0.89±0.07bc
    牡蛎酶解中剂量组 Oyster hydrolysis medium dose (OH-M) 2.60±0.49cd 3.43±0.25cd 0.78±0.06d
    牡蛎酶解高剂量组 Oyster hydrolysis high dose (OH-H) 1.40±0.49e 2.21±0.42e 0.65±0.02e
    注:每列不同字母代表差异显著 (P<0.05) Note: Different letters in the same column represent significant difference (P<0.05).
    下载: 导出CSV
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  • 收稿日期:  2020-07-07
  • 修回日期:  2020-08-21
  • 网络出版日期:  2020-11-16

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