留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

自然感染无乳链球菌罗非鱼的比较病理学及毒力基因谱分析

谢云丹 冯娟 刘婵 邓益琴 王江勇 苏友禄

引用本文:
Citation:

自然感染无乳链球菌罗非鱼的比较病理学及毒力基因谱分析

    作者简介: 谢云丹(1994—),女,硕士研究生,从事水产动物病原学研究。E-mail: 1159412450@qq.com;
    通讯作者: 苏友禄, suyoulu@scsfri.ac.cn
  • 中图分类号: S 941.42

Comparative pathological study of tilapia naturally infected with Streptococcus agalactiae and virulence gene profiling of isolated strains

    Corresponding author: Youlu SU, suyoulu@scsfri.ac.cn
  • CLC number: S 941.42

  • 摘要: 在自然感染无乳链球菌(Streptococcus agalactiae)的罗非鱼(Oreochromis niloticus)成鱼、稚鱼和自然携带无乳链球菌的罗非鱼体内分别获得14、4和2株无乳链球菌。临床和组织病理学分析显示,罗非鱼成鱼出现无规则游动,脑、眼眶、鳃和鳍条充血,眼球突出、白浊,内脏器官肿大、充血,以肾小管玻璃样变性、脑膜炎和心外膜炎等组织病理学变化为特征;罗非鱼稚鱼体表无明显症状,但部分内脏器官呈现肿大、充血现象,以脾脏血管区出血、肾小管上皮细胞变性、脑组织炎症反应较轻为其主要组织病理学特征。此外,罗非鱼胃固有层内及稚鱼肝脏组织中有大量的嗜酸性粒细胞浸润,可观察到无乳链球菌在成鱼的脑、心脏以及稚鱼肝脏中增殖;自然携带无乳链球菌的罗非鱼临床症状和组织学病变均不明显。PCR检测发现,各无乳链球菌毒力基因谱相同,但自然感染无乳链球菌的罗非鱼成鱼、稚鱼和自然携带无乳链球菌的罗非鱼的病理学损伤差异显著。
  • 图 1  罗非鱼脑组织PCR检测结果

    Figure 1.  PCR detection results of tilapia brain

    图 2  各株无乳链球菌感染斑马鱼后的累积死亡率

    Figure 2.  Cumulative motality of zebrafish infected with different S. agalactiae strains

    图 3  鱼感染无乳链球菌的眼观剖检变化

    Figure 3.  Change in anatomy of fish infected with S. agalactiae

    图 6  自然携带无乳链球菌的罗非鱼组织病理学

    Figure 6.  Histopathology of tilapia carrying S. agalactiae.

    图 4  自然感染无乳链球菌罗非鱼成鱼组织病理学

    Figure 4.  Histopathology of adult tilapia naturally infected with S. agalactiae

    图 5  自然感染无乳链球菌罗非鱼稚鱼组织病理学

    Figure 5.  Histopathology of juvenile tilapia naturally infected with S. agalactiae

    图 7  无乳链球菌21种毒力基因PCR扩增

    Figure 7.  PCR amplification of 21 virulence gene of S. agalactiae

    表 1  引物列表

    Table 1.  Primers of this study

    引物
    primer
    上游引物序列(5′−3′)
    forward primer sequence
    下游引物序列(5′−3′)
    reverse primer sequence
    扩增靶标
    amplification target
    长度/bp
    length
    AGAGTTTGATCC TGGCTCAG TACGGCTACCTTGTTACGACTT 16S rDNA 1 472
    sdi-F/R ATTCTCCTCCTGGCAAAGCC TGACGCTTGGTAGTTGCTGT 16s-23s rDNA 192
    fbsA-F/R AGTGTTGGAAATCAAAGTCAAGGT TTCATTGCGTCTCAAACCGC 纤维蛋白结合蛋白A (fbsA) 924
    cfb-F/R AACTCTAGTGGCTGGTGCAT CTCCAACAGCATGTGTGATTGC CAMP因子基因(cfb) 650
    dltR-F/R GTCTGAAGGTCCCCAAACCT TGTTACCCAAACGCTCAGGAT 调节蛋白基因(dltR) 392
    ponA-F/R ACAACTTGCTTTGCTCGCTG AGAGCCCTTCTGGCATTGTC 青霉素结合蛋白基因(ponA) 1 337
    hylB-F/R TCCACAACCCGTCACAACAC AACGCGCCCCATATCTACTA 透明质酸酶基因(hylB) 790
    cspA-F/R TGCACGTAACCAGTATCGCA GCACCGAGTTTAACGGCATC 丝氨酸蛋白酶基因(cspA) 175
    sodA-F/R TGATGCGCTTGAGCCACATA GCTTTGATGTAGTTAGGACGAACA 超氧化物歧化酶基因(sodA) 513
    sip-F/R ACAGATACGACGTGGACAGC ACCACGATCTGGCATTGCAT 表面免疫相关蛋白基因(sip) 1 173
    fbsB-F/R AGTTGCGCAAACTTCTGTCC TTTCCGCAGTTGTTACACCG 纤维蛋白结合蛋白B基因(fbsB) 158
    iagA-F/R GCATGGCCATTCCACTGAAG GCTAGCACTCATGGCACCTT 侵袭相关基因(iagA) 493
    scpB-F/R TGCGGCCTTTATCAGTCGAA AACAGTCCCATGATACCCGC C5a肽酶基因(scpB) 273
    bca-F/R TCAAGTTTGGTGCAGCTTCTG TCCGGTACTGACAATACTAACAAT αC蛋白基因(bca) 616
    srr-1-F/R ATGTTGCAGTAAAGCGCTGC GGAAGAGAGTCGTTTTCGGC 富含丝氨酸重复蛋白基因(srr-1) 727
    bibA-F/R TGCATAATATCCAGGTGTAGGCA TGAGAGATTGGGAAGTGGTGC 免疫原性细菌黏附蛋白基因(bibA) 943
    psaA-F/R AGCTGTCACCCTTTTGACCTT TAGGCTTAGGTGCCTGTGCT 肺炎球菌表面抗原A基因(psaA) 828
    lmb-F/R ATTTGTGACGCAACACACGG TCTTGTTTCCGCTTGGAGCA 层黏连蛋白结合蛋白基因(lmb) 263
    spb1-F/R GACATGGGGAGATGGTGGTG AGCTTCTGTGCCCCATTCAA 溶血素III (spb1) 652
    bac-F/R TGATTCCCTTTTGCTCTGCCA GTTCATGGGAAGCGTTGCTC βC蛋白基因(bac) 557
    pavA-F/R TCGACTTACATTGCCCCACC GGCGGCATCTGTCTTAACCT 纤维蛋白结合蛋白基因(pavA) 996
    cppA-F/R TGCAAATCTTGTCCCTGTGC TCGTACTCGTGCGGTGAATG C3降解蛋白酶基因(cppA) 387
    cylE-F/R ATTCTCCTCCTGGCAAAGCC TGACGCTTGGTAGTTGCTGT β-溶血素/溶细胞素基因(cylE) 176
    下载: 导出CSV

    表 2  样品采集和无乳链球菌菌株分离信息

    Table 2.  Sample collection and information of separation of S. agalactiae strain

    质量/g
    mass
    采样地
    sampling city
    养殖密度
    breeding density
    发病史
    historyof disease
    样品数(尾)
    number of samples (ind)
    菌株数
    number of strains
    菌株编号
    strain No.
    检出率
    detection rate
    ≈500 开平市 ≈100尾·hm–2 159 2 TKP1601-02 1.26%
    ≈15 高州市 ≈200尾·hm–2 爆发 7 4 TGZ1601-04 57.10%
    ≈500 廉江市 4 4 TLJ1601-04 63.64%
    吴川市 4 2 TWC1601-02
    惠州市 6 0
    河源市 8 8 TLC1601-08
    下载: 导出CSV

    表 3  21对毒力基因检测结果

    Table 3.  Detection results of 21 virulence genes

    毒力基因
    virulence gene
    菌株
    strain
    1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
    fbsA + + + + + + + + + + + + + + + + + + + +
    cfb + + + + + + + + + + + + + + + + + + + +
    dltR + + + + + + + + + + + + + + + + + + + +
    ponA + + + + + + + + + + + + + + + + + + + +
    hylB + + + + + + + + + + + + + + + + + + + +
    cspA + + + + + + + + + + + + + + + + + + + +
    sodA + + + + + + + + + + + + + + + + + + + +
    sip + + + + + + + + + + + + + + + + + + + +
    fbsB + + + + + + + + + + + + + + + + + + + +
    iagA + + + + + + + + + + + + + + + + + + + +
    scpB
    bca + + + + + + + + + + + + + + + + + + + +
    srr-1 + + + + + + + + + + + + + + + + + + + +
    bibA + + + + + + + + + + + + + + + + + + + +
    psaA + + + + + + + + + + + + + + + + + + + +
    lmb
    spb1 + + + + + + + + + + + + + + + + + + + +
    bac + + + + + + + + + + + + + + + + + + + +
    pavA + + + + + + + + + + + + + + + + + + + +
    cppA + + + + + + + + + + + + + + + + + + + +
    cylE + + + + + + + + + + + + + + + + + + + +
     注:1−2. TKP1601-02;3−6. TGZ1601-04;7−10. TLJ1601-04;11−12. TWC1601-02;13−20. TLC1601-08
    下载: 导出CSV
  • [1] 顾慧敏, 胡引. 1例分娩期合并无乳链球菌败血症患者的抢救及护理[J]. 中国实用护理杂志, 2014, 30(12): 56-57
    [2] SANZROJAS P, CABEZAOSORIO L, HERMOSA C, et al. Acute meningitis by Streptococcus agalactiae in a immunocompetent male[J]. Rev Esp Quim, 2013, 26(1): 78-79
    [3] JAWA G, HUSSAIN Z, Da S O. Recurrent late-onset group B Streptococcus sepsis in a preterm infant acquired by expressed breastmilk transmission: a case report[J]. Breastfeed Med, 2013, 8(1): 134-136 doi: 10.1089/bfm.2012.0016
    [4] VILLENA RUIZ M A, OLALLA S J, De L T L J, et al. Streptococcus agalactiae induced cavitated pneumonia[J]. Rev Clin Esp, 2009, 209(5): 252-254 doi: 10.1016/S0014-2565(09)71245-7
    [5] ELLIOTT J A, FACKLAM R R, RICHTER C B. Whole-cell protein patterns of nonhemolytic group B, type Ib, streptococci isolated from humans, mice, cattle, frogs, and fish[J]. J Clin Microbiol, 1990, 28(3): 628-630
    [6] EVANS J J, BOHNSACK J F, KLESIUS P H, et al. Phylogenetic relationships among Streptococcus agalactiae isolated from piscine, dolphin, bovine and human sources: a dolphin and piscine lineage associated with a fish epidemic in Kuwait is also associated with human neonatal infections in Japan[J]. J Med Microbiol, 2008, 57(11): 1369-1376 doi: 10.1099/jmm.0.47815-0
    [7] GENG Y, WANG K Y, HUANG X L, et al. Streptococcus agalactiae, an emerging pathogen for cultured ya-Fish, Schizothorax prenanti, in China[J]. Transbound Emerg Dis, 2012, 59(4): 369-375 doi: 10.1111/tbed.2012.59.issue-4
    [8] 崔静雯, 汪开毓, 贺扬, 等. 无乳链球菌感染尼罗罗非鱼的脑膜炎模型[J]. 水产学报, 2015, 39(12): 1883-1893
    [9] 王瑞, 李莉萍, 黄婷, 等. 罗非鱼组织内无乳链球菌实时荧光定量PCR检测方法建立[J]. 南方水产科学, 2015(3): 41-46 doi: 10.3969/j.issn.2095-0780.2015.03.007
    [10] 卢迈新, 黎炯, 叶星, 等. 广东与海南养殖罗非鱼无乳链球菌的分离、鉴定与特性分析[J]. 微生物学通报, 2010, 37(5): 766-774
    [11] HEMANDEZ E, FIGUEROA J, IREGUI C. Streptococcosis on a red tilapia, Oreochromis sp., farm: a case study[J]. J Fish Dis, 2009, 32(3): 247-252 doi: 10.1111/jfd.2009.32.issue-3
    [12] 祝璟琳, 杨弘. 鱼源无乳链球菌致病机理研究进展[J]. 广东海洋大学学报, 2013, 33(6): 92-96
    [13] CHIDEROLI R T, AMOROSO N, MAINARDI R M, et al. Emergence of a new multidrug-resistant and highly virulent serotype of Streptococcus agalactiae in fish farms from Brazil[J]. Aquaculture, 2017, 479: 45-51 doi: 10.1016/j.aquaculture.2017.05.013
    [14] 韦现色, 林勇, 杨慧赞, 等. 广西罗非鱼链球菌病的流行及防治[J]. 广西畜牧兽医, 2013, 29(1): 57-60 doi: 10.3969/j.issn.1002-5235.2013.01.031
    [15] 方伟, 梁宇恒, 宁丹, 等. 广东地区感染养殖罗非鱼的无乳链球菌分子分型研究[J]. 中山大学学报(自然科学版), 2016, 55(2): 97-101
    [16] SU Y L, FENG J, LIU C, et al. Dynamic bacterial colonization and microscopic lesions in multiple organs of tilapia infected with low and high pathogenic Streptococcus agalactiae strains[J]. Aquaculture, 2017, 471: 190-203 doi: 10.1016/j.aquaculture.2017.01.013
    [17] PATRAS K A, NIZET V. Group B Streptococcal maternal colonization and neonatal disease: molecular mechanisms and preventative approaches[J]. Front Pediatr, 2018, 6: 27 doi: 10.3389/fped.2018.00027
    [18] LECLERCQ S Y, SULLIVAN M J, IPE D S, et al. Pathogenesis of Streptococcus urinary tract infection depends on bacterial strain and β-hemolysin/cytolysin that mediates cytotoxicity, cytokine synthesis, inflammation and virulence[J]. Sci Rep-UK, 2016, 6: 29000 doi: 10.1038/srep29000
    [19] GENDRIN C, LEMBO A, WHIDBEY C, et al. The sensor histidine kinase RgfC affects group B Streptococcal virulence factor expression independent of its response regulator RgfA[J]. Infect Immun, 2015, 83(3): 1078-1088 doi: 10.1128/IAI.02738-14
    [20] 李庆勇, 可小丽, 卢迈新, 等. 罗非鱼无乳链球菌C5a肽酶(ScpB)的原核表达及其免疫原性[J]. 中国水产科学, 2014, 21(1): 169-179
    [21] 曾祖聪, 可小丽, 卢迈新, 等. 罗非鱼无乳链球菌LrrG-Sip融合蛋白免疫原性研究[J]. 南方水产科学, 2017, 13(3): 51-57 doi: 10.3969/j.issn.2095-0780.2017.03.007
    [22] ZHANG D, LI A, GUO Y, et al. Molecular characterization of Streptococcus agalactiae in diseased farmed tilapia in China[J]. Aquaculture, 2013, 412(6): 64-69
    [23] KANNIKA K, PISUTTHARACHAI D, SRISAPOOME P, et al. Molecular serotyping, virulence gene profiling and pathogenicity of Streptococcus agalactiae isolated from tilapia farms in Thailand by multiplex PCR[J]. J APPL MICROBIOL, 2017, 122(6): 1497-1507 doi: 10.1111/jam.2017.122.issue-6
    [24] BERRIDGE B R, BERCOVIER H, FRELIER P F. Streptococcus agalactiae and Streptococcus difficile 16S-23S intergenic rDNA: genetic homogeneity and species-specific PCR[J]. Vet Microbiol, 2001, 78(2): 165-173 doi: 10.1016/S0378-1135(00)00285-6
    [25] PATTERSON H, SARALAHTI A, PARIKKA M, et al. Adult zebrafish model of bacterial meningitis in Streptococcus agalactiae infection[J]. Dev Comp Immunol, 2012, 38(3): 447-455 doi: 10.1016/j.dci.2012.07.007
    [26] KAYANSAMRUAJ P, PIRARAT N, KATAGIRI T, et al. Molecular characterization and virulence gene profiling of pathogenic Streptococcus agalactiae populations from tilapia (Oreochromis sp.) farms in Thailand[J]. J Vet Diagn Invest, 2014, 26(4): 488 doi: 10.1177/1040638714534237
    [27] GODOY D T, CARVALHOCASTRO G A, LEAL C A, et al. Genetic diversity and new genotyping scheme for fish pathogenic Streptococcus agalactiae[J]. Lett Appl Microbiol, 2013, 57(6): 476-483 doi: 10.1111/lam.2013.57.issue-6
    [28] UDO E E, BOSWIHI S S, ALSWEIH N. Genotypes and virulence genes in group B Streptococcus isolated in the maternity hospital, Kuwait[J]. Med Prin Pract, 2013, 22(5): 453-457 doi: 10.1159/000349932
    [29] RAJAGOPAL L. Understanding the regulation of group B Streptococcal virulence factors[J]. Future Microbiol, 2009, 4(2): 201-221 doi: 10.2217/17460913.4.2.201
    [30] EVANS J J, PASNIK D J, KLESIUS P H. Differential pathogenicity of five Streptococcus agalactiae isolates of diverse geographic origin in Nile tilapia (Oreochromis niloticus L.)[J]. Aquac Res, 2015, 46(10): 2374-2381 doi: 10.1111/are.2015.46.issue-10
    [31] 祝璟琳, 邹芝英, 李大宇, 等. 尼罗罗非鱼无乳链球菌病的病理学研究[J]. 水产学报, 2014, 38(11): 1937-1944
    [32] ABUSELIANA A F, DAUD H H M, AZIZ S A, et al. Pathogenicity of Streptococcus agalactiae isolated from a fish farm in selangor to juvenile red tilapia (Oreochromis sp.)[J]. J Anim Vet Adv, 2011, 10(7): 914-919 doi: 10.3923/javaa.2011.914.919
    [33] 姜建强, 额尔敦木图, 包花尔, 等. 嗜酸性粒细胞与寄生虫感染免疫的相关性[J]. 黑龙江畜牧兽医, 2016(2): 63-67
    [34] REIMERT C M, FITZSIMMONS C M, JOSEPH S, et al. Eosinophil activity in Schistosoma mansoni infections in vivo and in vitro in relation to plasma cytokine profile pre- and posttreatment with praziquantel[J]. Clin Vaccine Immunol, 2006, 13(5): 584-593 doi: 10.1128/CVI.13.5.584-593.2006
    [35] LIN P Y, LAN R, SINTCHENKO V, et al. Computational bacterial genome-wide analysis of phylogenetic profiles reveals potential virulence genes of Streptococcus agalactiae[J]. Plos One, 2011, 6(4): e17964 doi: 10.1371/journal.pone.0017964
    [36] UDO E E, BOSWIHI S S, ALSWEIH N. Genotypes and virulence genes in group B Streptococcus isolated in the maternity hospital, Kuwait[J]. Med Prin Pract, 2013, 22(5): 453-457 doi: 10.1159/000349932
    [37] DORAN K S, LIU G Y, NIZET V. Group B Streptococcal beta-hemolysin/cytolysin activates neutrophil signaling pathways in brain endothelium and contributes to development of meningitis[J]. J Clin Invest, 2003, 112(5): 736-744 doi: 10.1172/JCI200317335
    [38] RING A, BRAUN J S, POHL J, et al. Group B Streptococcal beta-hemolysin induces mortality and liver injury in experimental sepsis[J]. J Infect Dis, 2002, 185(12): 1745-1753 doi: 10.1086/jid.2002.185.issue-12
    [39] CHENG Q, CARLSON B, PILLAI S, et al. Antibody against Surface-Bound C5a peptidase is opsonic and initiates macrophage killing of group B Streptococci[J]. Infect Immun, 2001, 69(4): 2302-2308 doi: 10.1128/IAI.69.4.2302-2308.2001
    [40] BOHNSACK J F, WIDJAJA K, GHAZIZADEH S, et al. A role for C5 and C5a-ase in the acute neutrophil response to group B Streptococcal infections[J]. J Infect Dis, 1997, 175(4): 847-855 doi: 10.1086/jid.1997.175.issue-4
    [41] 胡会杰, 张琪, 周明旭, 等. 不同禽源致病性大肠杆菌毒力基因分布规律研究[J]. 中国家禽, 2015, 37(10): 34-37
    [42] WANG Z, GUO C, XU Y, et al. Two novel functions of hyaluronidase from Streptococcus agalactiae are enhanced intracellular survival and inhibition of proinflammatory cytokine expression[J]. INFECT IMMUN, 2014, 82(6): 2615 doi: 10.1128/IAI.00022-14
    [43] BACHRACH G, ZLOTKIN A, HURVITZ A, et al. Recovery of Streptococcus iniae from diseased fish previously vaccinated with a Streptococcus vaccine[J]. Appl Environ Micro, 2001, 67(8): 3756 doi: 10.1128/AEM.67.8.3756-3758.2001
    [44] ABUSELIANA A F, DAUD H H M, AZIZ S A, et al. Pathogenicity of Streptococcus agalactiae isolated from a fish farm in selangor to juvenile red tilapia (Oreochromis sp.)[J]. J ANIM VET ADV, 2011, 10(7): 914-919 doi: 10.3923/javaa.2011.914.919
    [45] ALHARBI A H. Phenotypic and genotypic characterization of Streptococcus agalactiae isolated from hybrid tilapia (Oreochromis niloticus×O. aureus)[J]. Aquaculture, 2016, 464: 515-520 doi: 10.1016/j.aquaculture.2016.07.036
    [46] ELDAR A, BEJERANO Y, LIVOFF A, et al. Experimental Streptococcal meningo-encephalitis in cultured fish[J]. Vet Microbiol, 1995, 43(1): 33-40 doi: 10.1016/0378-1135(94)00052-X
  • [1] 曾祖聪曹建萌卢迈新可小丽刘志刚高风英朱华平 . 罗非鱼无乳链球菌LrrG-Sip融合基因原核表达载体的构建及表达. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2014.05.003
    [2] 马艳平李嘉彬郝乐刘振兴冯国清周结珊柯浩 . 罗非鱼源无乳链球菌S-核糖基高半胱氨酸酶基因(luxS)的克隆及其推导蛋白的三维结构预测. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2013.01.007
    [3] 刘志刚可小丽卢迈新方伟王淼朱华平高风英曹建萌 . 罗非鱼无乳链球菌强毒株基因组表达文库的构建及鉴定. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2015.06.005
    [4] 王瑞李莉萍黄婷梁万文梁聪雷爱莹陈明 . 罗非鱼组织内无乳链球菌实时荧光定量PCR检测方法建立. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2015.03.007
    [5] 曾祖聪可小丽卢迈新刘志刚曹建萌高风英朱华平王淼 . 罗非鱼无乳链球菌LrrG-Sip融合蛋白免疫原性研究. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2017.03.007
    [6] 高铭蔚黎宗强田园园叶星孙成飞董浚键卢迈新 . 无乳链球菌乳酸-羟基乙酸共聚物微球的制备及其体外释放特点分析. 南方水产科学, doi: 10.3969/j.issn2095-0780.2014.03.010
    [7] 马茹飞黄长江 . 奥尼罗非鱼壬基酚慢性中毒的病理学研究. 南方水产科学,
    [8] 卢迈新 . 罗非鱼链球菌病研究进展. 南方水产科学, doi: 10.3969/j.issn.1673-2227.2010.01.013
    [9] 吴南郭佳熊丽张永安 . 罗非鱼链球菌病疫苗的研究进展. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2013.06.015
    [10] 朱佳杰李莉萍唐瞻杨周宇罗永巨甘西 . 吉富罗非鱼家系构建及抗病力检测. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2012.06.004
    [11] 刘玉姣朱华平卢迈新刘志刚曹建萌高风英可小丽 . 罗非鱼催乳素I基因的组织分布及盐胁迫对其表达的影响. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2014.06.008
    [12] 马庆男董在杰朱文彬苏胜彦张宁袁新华杨弘 . 罗非鱼TRAP分子标记反应体系优化设计方案的比较. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2013.01.005
    [13] 曾少葵杨萍陈秀红 . 微生物发酵对罗非鱼下脚料蛋白酶解液脱腥去苦效果比较. 南方水产科学, doi: 10.3969/j.issn.1673-2227.2009.04.011
    [14] 冯敬宾贾晓平 . 2种水温条件下罗非鱼体内氟苯尼考的药物动力学比较. 南方水产科学,
    [15] 李富祥王鹏飞闫路路邱丽华 . 花鲈irak4基因cDNA的克隆与表达分析. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2018.05.009
    [16] 刘在军岑剑伟李来好杨贤庆郝淑贤魏涯周婉君 . 罗非鱼血液综合利用的研究思路及展望. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2012.02.012
    [17] 方静黄卉李来好杨贤庆郝淑贤魏涯岑剑伟戚勃 . 不同致死方式对罗非鱼鱼片品质的影响. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2013.05.003
    [18] 段志刚吴金英李文笙 . 低温对罗非鱼类影响的相关研究进展. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2011.06.013
    [19] 赵志霞吴燕燕李来好陈胜军林婉玲邓建朝 . 低盐罗非鱼片快速腌制的工艺研究. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2017.06.013
    [20] 吴燕燕赵志霞李来好郝淑贤邓建朝胡晓 . 添加外源酶类对腌制罗非鱼品质的影响. 南方水产科学, doi: 10.3969/j.issn.2095-0780.2018.04.013
  • 加载中
计量
  • 文章访问数:  101
  • 被引次数: 0
出版历程
  • 收稿日期:  2018-08-17
  • 录用日期:  2018-11-05
  • 网络出版日期:  2018-12-25

自然感染无乳链球菌罗非鱼的比较病理学及毒力基因谱分析

    作者简介:谢云丹(1994—),女,硕士研究生,从事水产动物病原学研究。E-mail: 1159412450@qq.com
    通讯作者: 苏友禄, suyoulu@scsfri.ac.cn
  • 1. 天津农学院水产学院 天津 300384
  • 2. 中国水产科学研究院南海水产研究所,农业农村部南海渔业资源开发利用重点实验室 广东 广州 510300

摘要: 在自然感染无乳链球菌(Streptococcus agalactiae)的罗非鱼(Oreochromis niloticus)成鱼、稚鱼和自然携带无乳链球菌的罗非鱼体内分别获得14、4和2株无乳链球菌。临床和组织病理学分析显示,罗非鱼成鱼出现无规则游动,脑、眼眶、鳃和鳍条充血,眼球突出、白浊,内脏器官肿大、充血,以肾小管玻璃样变性、脑膜炎和心外膜炎等组织病理学变化为特征;罗非鱼稚鱼体表无明显症状,但部分内脏器官呈现肿大、充血现象,以脾脏血管区出血、肾小管上皮细胞变性、脑组织炎症反应较轻为其主要组织病理学特征。此外,罗非鱼胃固有层内及稚鱼肝脏组织中有大量的嗜酸性粒细胞浸润,可观察到无乳链球菌在成鱼的脑、心脏以及稚鱼肝脏中增殖;自然携带无乳链球菌的罗非鱼临床症状和组织学病变均不明显。PCR检测发现,各无乳链球菌毒力基因谱相同,但自然感染无乳链球菌的罗非鱼成鱼、稚鱼和自然携带无乳链球菌的罗非鱼的病理学损伤差异显著。

English Abstract

相关文章 (20)

返回顶部

目录

    /

    返回文章
    返回