Response surface methodology for optimization of sterilization effect on tilapia fillet with slightly acidic electrolyzed water
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摘要: 为研究微酸性电解水对新鲜罗非鱼片的杀菌效果,以杀菌对数值为指标,进行单因素和响应面实验,建立各因素与响应值之间的数学模型,确定最佳杀菌条件。结果表明,最佳杀菌条件为有效氯质量浓度35.00 mg·L–1,浸泡时间22 min,料液质量体积比1∶6,在此处理条件下,杀菌对数值为(0.735±0.001) lg (CFU·g–1),杀菌率为(81.59±0.04)%。Abstract: In order to study the sterilization effect of fresh tilapia fillets with slightly acidic electrolyzed water, the single factor and response surface experiment were adopted to establish the mathematical model between the factors and response values, and the best sterilization conditions were determined. The results show that the best bactericidal condition was 35.00 mg·L–1 of available chlorine concentration, 22 min for soaking time and 1∶6 (m∶V ). Under this condition, the killing logarithm value of bacterial colony was (0.735±0.001) lg (CFU·g–1), and the bactericidal rate was (81.59±0.04)%.
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Key words:
- tilapia fillet /
- slightly acidic electrolyzed water /
- response surface /
- sterilization
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表 1 微酸性电解水的理化特性
Table 1. Physico-chemical properties of slightly acidic electrolyzed water
有效氯质量浓度/mg·L–1
ACCpH 氧化还原电位值/mV
ORP12.48±0.47 6.39±0.06 882.93±9 19.85±1.71 6.16±0.09 898.54±19.03 31.39±1.48 6.053 3±0.19 900.63±14.3 35.22±0.98 5.912±0.07 906.43±12.12 40.270 4±1.36 5.895±0.09 910.23±15.83 49.24±1.24 5.33±0.08 950.43±13.23 表 2 响应面实验设计因素与水平
Table 2. Factors and levels used in response surface experiments
水平
level因素 factor 有效氯质量浓度/mg·L–1
available chlorine concentration浸泡时间/min
dipping time料液质量体积比
solid-liquid ratio–1 20 15 1∶4 0 30 20 1∶6 1 40 25 1∶8 表 3 Box-Behnken实验方案及结果
Table 3. Box-Behnken design and results
实验号
test No.有效氯浓度
available chlorine concentration浸泡时间
dipping time料液比
solid-liquid ratio杀菌对数值
lg colony killing value1 –1 –1 0 0.52 2 1 –1 0 0.66 3 –1 1 0 0.64 4 1 1 0 0.7 5 –1 0 –1 0.56 6 1 0 –1 0.67 7 –1 0 1 0.6 8 1 0 1 0.69 9 0 –1 –1 0.59 10 0 1 –1 0.65 11 0 –1 1 0.61 12 0 1 1 0.68 13 0 0 0 0.73 14 0 0 0 0.71 15 0 0 0 0.69 16 0 0 0 0.72 17 0 0 0 0.74 表 4 回归方程方差分析
Table 4. Analysis of variances for developed regression equation
方差来源
source平方和
SS自由度
df均方
MSF P 显著性
significance模型 model 0.061 9 0.006 29.36 <0.000 1 ** A-有效氯浓度 A-available chlorine concentration 0.02 1 0.02 87.23 <0.000 1 ** B-浸泡时间 B-dipping time 0.011 1 0.011 45.85 0.000 3 ** C-料液比 C-solid-liquid ratio 0.001 1 0.001 5 6.6 0.037 1 * AB 0.001 1 0.001 6 6.98 0.033 3 * AC 0.000 1 1 0.000 1 0.44 0.530 1 BC 0.000 025 1 0.000 02 0.11 0.750 9 A2 0.008 1 0.008 6 37.6 0.000 5 ** B2 0.007 1 0.007 6 33.56 0.000 7 ** C2 0.007 1 0.007 6 33.56 0.000 7 ** 残差 residual 0.001 7 0.000 2 失拟项 lack of fit 0.000 1 3 0.000 04 0.11 0.948 2 误差项 pure error 0.001 4 0.000 3 总和 cor total 0.062 16 R2=0.974 1 RAdj=0.941 0 注:*. P<0.05表示差异显著;**. P<0.01表示差异极显著 Note: *. significant difference (P<0.05); **. very significant difference (P<0.01) -
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