罗非鱼片微酸性电解水杀菌工艺响应面法优化研究

于福田; 岑剑伟; 李来好; 杨贤庆; 黄卉; 郝淑贤; 魏涯; 赵永强; 林织

doi:10.12131/20180164

罗非鱼片微酸性电解水杀菌工艺响应面法优化研究

doi: 10.12131/20180164

于福田^{1, 2,},
岑剑伟^1, ,,
李来好¹,
杨贤庆^1,,
黄卉¹,
郝淑贤¹,
魏涯¹,
赵永强¹,
林织³

1.
中国水产科学研究院南海水产研究所，农业农村部水产品加工重点实验室，广东广州 510300
2.
上海海洋大学食品学院，上海 201306
3.
广东顺欣海洋渔业集团有限公司，广东阳江 529800

基金项目: 中国水产科学研究院基本科研业务费资助项目 (2016HY-ZD1001)；现代农业 (罗非鱼) 产业技术体系建设专项资金 (CARS-46)；“扬帆计划”引进创新创业团队专项资助 (2015YT02H109)；国家自然科学基金项目(31601533)

详细信息

作者简介:
于福田(1992—)，男，硕士研究生，从事水产品加工研究。E-mail: yufutian2012@163.com

通讯作者:
岑剑伟 (1976 — ) ，男，副研究员，从事水产品加工及质量安全研究。E-mail: genvex@163.com

中图分类号: S 983.09.19
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出版历程
- 收稿日期: 2018-07-17
- 修回日期: 2018-09-10
- 网络出版日期: 2018-12-05
- 刊出日期: 2019-02-05

Response surface methodology for optimization of sterilization effect on tilapia fillet with slightly acidic electrolyzed water

1.
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
2.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
3.
Guangdong Shun Xin Ocean Fishery Group Co., Ltd., Yangjiang 529800, China

摘要

摘要: 为研究微酸性电解水对新鲜罗非鱼片的杀菌效果，以杀菌对数值为指标，进行单因素和响应面实验，建立各因素与响应值之间的数学模型，确定最佳杀菌条件。结果表明，最佳杀菌条件为有效氯质量浓度35.00 mg·L^–1，浸泡时间22 min，料液质量体积比1∶6，在此处理条件下，杀菌对数值为(0.735±0.001) lg (CFU·g^–1)，杀菌率为(81.59±0.04)%。
- 罗非鱼片 /
- 微酸性电解水 /
- 响应面 /
- 杀菌
Abstract: In order to study the sterilization effect of fresh tilapia fillets with slightly acidic electrolyzed water, the single factor and response surface experiment were adopted to establish the mathematical model between the factors and response values, and the best sterilization conditions were determined. The results show that the best bactericidal condition was 35.00 mg·L^–1 of available chlorine concentration, 22 min for soaking time and 1∶6 (m∶V ). Under this condition, the killing logarithm value of bacterial colony was (0.735±0.001) lg (CFU·g^–1), and the bactericidal rate was (81.59±0.04)%.
- tilapia fillet /
- slightly acidic electrolyzed water /
- response surface /
- sterilization

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图 1 微酸性电解水有效氯浓度对杀菌效果的影响

Figure 1. Effect of slightly acidic electrolyzed water available chlorine concentration on sterilization

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图 2 微酸性电解水浸泡时间对杀菌效果的影响

Figure 2. Effect of slightly acidic electrolyzed water dipping time on sterilization

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图 3 罗非鱼片与酸性电解水料液比对杀菌效果的影响

Figure 3. Effect of tilapia fillet and slightly acidic electrolyzed water's solid-liquid ratio on sterilization

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图 4 Y=(A，B) 的响应面与等高线

Figure 4. Response surface and contour plots under Y=(A, B)

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图 5 Y=(A，C) 的响应面与等高线

Figure 5. Response surface and contour plots under Y=(A, C)

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图 6 Y=(B，C) 的响应面与等高线

Figure 6. Response surface and contour plots under Y=(B, C)

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表 1 微酸性电解水的理化特性

Table 1. Physico-chemical properties of slightly acidic electrolyzed water

有效氯质量浓度/mg·L^–1 ACC	pH	氧化还原电位值/mV ORP
12.48±0.47	6.39±0.06	882.93±9
19.85±1.71	6.16±0.09	898.54±19.03
31.39±1.48	6.053 3±0.19	900.63±14.3
35.22±0.98	5.912±0.07	906.43±12.12
40.270 4±1.36	5.895±0.09	910.23±15.83
49.24±1.24	5.33±0.08	950.43±13.23

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表 2 响应面实验设计因素与水平

Table 2. Factors and levels used in response surface experiments

水平 level	因素 factor
水平 level	有效氯质量浓度/mg·L^–1 available chlorine concentration	浸泡时间/min dipping time	料液质量体积比 solid-liquid ratio
–1	20	15	1∶4
0	30	20	1∶6
1	40	25	1∶8

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表 3 Box-Behnken实验方案及结果

Table 3. Box-Behnken design and results

实验号 test No.	有效氯浓度 available chlorine concentration	浸泡时间 dipping time	料液比 solid-liquid ratio	杀菌对数值 lg colony killing value
1	–1	–1	0	0.52
2	1	–1	0	0.66
3	–1	1	0	0.64
4	1	1	0	0.7
5	–1	0	–1	0.56
6	1	0	–1	0.67
7	–1	0	1	0.6
8	1	0	1	0.69
9	0	–1	–1	0.59
10	0	1	–1	0.65
11	0	–1	1	0.61
12	0	1	1	0.68
13	0	0	0	0.73
14	0	0	0	0.71
15	0	0	0	0.69
16	0	0	0	0.72
17	0	0	0	0.74

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表 4 回归方程方差分析

Table 4. Analysis of variances for developed regression equation

方差来源 source	平方和 SS	自由度 df	均方 MS	F	P	显著性 significance
模型 model	0.061	9	0.006	29.36	<0.000 1	**
A-有效氯浓度 A-available chlorine concentration	0.02	1	0.02	87.23	<0.000 1	**
B-浸泡时间 B-dipping time	0.011	1	0.011	45.85	0.000 3	**
C-料液比 C-solid-liquid ratio	0.001	1	0.001 5	6.6	0.037 1	*
AB	0.001	1	0.001 6	6.98	0.033 3	*
AC	0.000 1	1	0.000 1	0.44	0.530 1
BC	0.000 025	1	0.000 02	0.11	0.750 9
A²	0.008	1	0.008 6	37.6	0.000 5	**
B²	0.007	1	0.007 6	33.56	0.000 7	**
C²	0.007	1	0.007 6	33.56	0.000 7	**
残差 residual	0.001	7	0.000 2
失拟项 lack of fit	0.000 1	3	0.000 04	0.11	0.948 2
误差项 pure error	0.001	4	0.000 3
总和 cor total	0.062	16
	R²=0.974 1		R_Adj=0.941 0
注：. P<0.05表示差异显著；. P<0.01表示差异极显著　Note: . significant difference (P<0.05); **. very significant difference (P<0.01)

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参考文献(25)

[1]	李娜, 赵永强, 李来好, 等. 冰藏过程中罗非鱼鱼片肌肉蛋白质变化[J]. 南方水产科学, 2016, 12(2): 88-94. doi: 10.3969/j.issn.2095-0780.2016.02.013
[2]	于福田, 岑剑伟, 杨贤庆, 等. 罗非鱼片发色新技术研究进展[J]. 食品工业科技, 2018, 39(6): 322-326.
[3]	赵良, 岑剑伟, 李来好, 等. 高压静电场结合冰温气调保鲜技术对罗非鱼鱼片品质的影响[J]. 南方水产科学, 2016, 12(3): 91-97. doi: 10.3969/j.issn.2095-0780.2016.03.012
[4]	DEWI F R, STANLEY R, POWELL S M, et al. Application of electrolysed oxidising water as a sanitiser to extend the shelf-life of seafood products: a review[J]. J Food Sci Tech Mys, 2017, 54(5): 1321-1332. doi: 10.1007/s13197-017-2577-9
[5]	中村悌一. 微酸性电解水在食品厂卫生管理方面的应用[J]. 中国洗涤用品工业, 2017(1): 14-19.
[6]	堀田国元. 酸性电解水的基础、应用及发展动向[J]. 中国护理管理, 2008, 8(4): 7-11. doi: 10.3969/j.issn.1672-1756.2008.04.002
[7]	ISSA-ZACHARIA A, KAMITANI Y, MIWA N A, et al. Application of slightly acidic electrolyzed water as a potential non-thermal food sanitizer for decontamination of fresh ready-to-eat vegetables and sprouts[J]. Food Control, 2011, 22(3/4): 601-607.
[8]	LIAO X Y, XUAN X T, LI J, et al. Bactericidal action of slightly acidic electrolyzed water against Escherichia coli and Staphylococcus aureus via multiple cell targets[J]. Food Control, 2017, 79: 380-385. doi: 10.1016/j.foodcont.2017.03.050
[9]	MANSUR A R, OH D H. Combined effects of thermosonication and slightly acidic electrolyzed water on the microbial quality and shelf life extension of fresh-cut kale during refrigeration storage[J]. Food Microbiol, 2015, 51: 154-162. doi: 10.1016/j.fm.2015.05.008
[10]	TANGO C N, MANSUR A R, KIM G H, et al. Synergetic effect of combined fumaric acid and slightly acidic electrolysed water on the inactivation of food-borne pathogens and extending the shelf life of fresh beef[J]. J Appl Microbiol, 2014, 117(6): 1709-1720. doi: 10.1111/jam.2014.117.issue-6
[11]	HUANG Y R, HUNG Y C, HSU S Y, et al. Application of electrolyzed water in the food industry[J]. Food Control, 2008, 19(4): 329-345. doi: 10.1016/j.foodcont.2007.08.012
[12]	JO H Y, TANGO C N, OH D H. Influence of different organic materials on chlorine concentration and sanitization of slightly acidic electrolyzed water[J]. LWT, 2018, 92: 187-194. doi: 10.1016/j.lwt.2018.02.028
[13]	WALKER S P, DEMIRCI A, GRAVES R E, et al. Response surface modelling for cleaning and disinfecting materials used in milking systems with electrolysed oxidizing water[J]. Int J Dairy Technol, 2005, 58(2): 65-73. doi: 10.1111/idt.2005.58.issue-2
[14]	TOMÁS-CALLEJAS A, LÓPEZ-GÁLVEZ F, SBODIO A, et al. Chlorine dioxide and chlorine effectiveness to prevent Escherichia coli O157:H7 and Salmonella cross-contamination on fresh-cut Red Chard[J]. Food Control, 2012, 23(2): 325-332. doi: 10.1016/j.foodcont.2011.07.022
[15]	ALEXANDRE E M, BRANDAO T R, SILVA C L. Assessment of the impact of hydrogen peroxide solutions on microbial loads and quality factors of red bell peppers, strawberries and watercress[J]. Food Control, 2012, 27(2): 362-368. doi: 10.1016/j.foodcont.2012.04.012
[16]	ALEXANDRE E M, SANTOS-PEDRO D M, BRANDAO T R, et al. Influence of aqueous ozone, blanching and combined treatments on microbial load of red bell peppers, strawberries and watercress[J]. J Food Eng, 2011, 105(2): 277-282. doi: 10.1016/j.jfoodeng.2011.02.032
[17]	HUANG Y X, CHEN H Q. Effect of organic acids, hydrogen peroxide and mild heat on inactivation of Escherichia coli O157:H7 on baby spinach[J]. Food Control, 2011, 22(8): 1178-1183. doi: 10.1016/j.foodcont.2011.01.012
[18]	YE Z Y, WANG S, CHEN T, et al. Inactivation mechanism of Escherichia coli induced by slightly acidic electrolyzed water[J]. Sci Rep, 2017, 7: 6279. doi: 10.1038/s41598-017-06716-9
[19]	李国威, 傅润泽, 沈建, 等. 微酸性电解水对活品虾夷扇贝存活率的影响及杀菌效果[J]. 渔业现代化, 2016, 43(1): 68-74. doi: 10.3969/j.issn.1007-9580.2016.01.013
[20]	唐鸟林. 电解水处理水产品及其加工环境中致病菌的研究[D]. 上海: 华东理工大学, 2010: 9-10.
[21]	赵德锟, 李凌飞, 谭雷姝, 等. 微酸性电解水对鲜切云南红梨贮藏品质影响研究[J]. 食品安全质量检测学报, 2017, 8(1): 243-251.
[22]	叶章颖, 祁凡雨, 裴洛伟, 等. 微酸性电解水对虾仁的杀菌效果及其动力学[J]. 农业工程学报, 2014, 30(3): 223-230.
[23]	王潇, 吴佳佳, 张鹤, 等. 响应面法优化中华管鞭虾酸性电解水杀菌工艺[J]. 中国食品学报, 2015(12): 107-114.
[24]	周小双, 王锦旭, 杨贤庆, 等. 响应面法优化合浦珠母贝糖胺聚糖提取工艺[J]. 食品与发酵工业, 2016, 42(1): 238-243.
[25]	赵中辉. 加工鱼块氯残留量检测及风险分析[J]. 检验检疫学刊, 2012, 22(5): 44-65. doi: 10.3969/j.issn.1674-5354.2012.05.014